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Oasis hlb reverse phase cartridges

Manufactured by Waters Corporation
Sourced in United States

OASIS HLB reverse-phase cartridges are a type of solid-phase extraction (SPE) media designed for the purification and concentration of a wide range of analytes from aqueous samples. The cartridges feature a hydrophilic-lipophilic balanced (HLB) sorbent that can retain both polar and non-polar compounds. They are commonly used for sample preparation prior to analysis by techniques such as high-performance liquid chromatography (HPLC) or mass spectrometry.

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2 protocols using oasis hlb reverse phase cartridges

1

HIV-1 p24 Antigen Detection Assay

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FTC was purchased from HBCChem (Union City, CA, USA). Chemicals, reagents, media and buffers used for chemical reactions or in vitro studies were purchased from either Sigma Aldrich (St. Louis, MO, USA) or Fisher Scientific (Waltham, MA, USA). Thin-layer chromatography was performed on precoated F-254 silica plates (250 µm) from SiliCycle, Inc. (Quebec City, QC, Canada). Monoclonal mouse anti-human HIV-1 p24 (clone Kal-1), and the polymer-based HRP-conjugated anti-mouse EnVision+ secondary antibodies were purchased from Dako (Carpinteria, CA, USA). MTT was purchased from Sigma Aldrich. FTC-TP was purchased from Toronto Research Chemicals (North York, ON, Canada). Sep-Pak QMA anion-exchange cartridges and OASIS HLB reverse-phase cartridges were purchased from Waters Corp. (Milford, MA, USA).
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2

Quantification of Nucleoside Analog Triphosphates

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3TC-TP, CBV-TP, CBV, 15N213C-3TC, d4-ABC, and emtricitabine-TP (FTC-TP) were obtained from Toronto Research Chemicals (North York, Canada). 3TC and ABC were provided by GlaxoSmithKline Inc. (Research Triangle Park, NC). Type XA acid phosphatase (EC 3.1.3.2) from sweet potatoes, guanosine, guanosine monophosphate (GMP), guanosine diphosphate (GDP), guanosine triphosphate (GTP), cytidine, cytidine monophosphate (CMP), cytidine diphosphate (CDP), and cytidine triphosphate (CTP) were purchased from Sigma Chemical Co. (St. Louis, MO). Sep-Pak QMA anion-exchange cartridges and OASIS HLB reverse-phase cartridges were purchased from Waters Corp. (Milford, MA). Potassium chloride (KCl) was purchased from JT Baker Co. (Phillipsburg, NJ). LC-MS grade (Optima) water (H2O), acetonitrile (ACN), methanol (MeOH), acetic acid, ammonium hydroxide, and ammonium bicarbonate were purchased from Fisher chemicals (Fair Lawn, NJ). RPMI media 1640, phosphate buffered saline (PBS), Hanks’ Balanced salt solution (HBSS), and fetal bovine serum were purchased from Invitrogen (Gibco; Carlsbad, CA).
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