Horseradish peroxidase hrp conjugated goat anti mouse igg

Recombinant human TGF-β1 was purchased from PeproTech (Princeton, NJ, USA). SB431542 was purchased from Selleckchem (Houston, TX, USA). RGD peptide was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). TGF-β neutralizing antibody was purchased from R&D Systems (Minneapolis, MN, USA). Anti-TNC antibodies for IHC staining and western blot analysis were purchased from Santa Cruz Biotechnology and Abcam (Cambridge, UK), respectively. Anti-GAPDH antibody was from AbClone (Seoul, Korea). Anti-SMAD2 (SMAD family member 2), anti-phospho-SMAD2, anti-SMAD3, and anti-phospho-SMAD3 antibodies were from Cell Signaling Technology (Danvers, MA, USA). Anti-pro-COL1A1 and anti-MMP-1 antibodies have been described previously [49 (link)]. Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG, goat anti-rabbit IgG, and rabbit anti-goat IgG antibodies were purchased from KOMA Biotech (Seoul, Korea). Rhodamine Red-X-conjugated goat anti-mouse IgG and Alexa Fluor 488-conjugated rabbit anti-goat IgG antibodies were purchased from Invitrogen (Carlsbad, CA, USA).

Recombinant human TNF-α was purchased from Peprotech and 2′,7′-dichlorofluorescein diacetate (DCF-DA) was purchased from Sigma–Aldrich. The following antibodies were used: anti-SOD3 for western blotting (ab83108) and anti-SOD3 for immunohistochemistry (IHC; ab171738) from Abcam; anti-glyceraldehyde dehydrogenase (GAPDH; ab83108) from AbClone; anti-phospho-NF-κB inhibitor protein ɑ (IκBɑ), anti-phospho-NF-κB p65, anti-phospho-c-jun N-terminal kinase (JNK), anti-JNK, anti-phospho-p38 mitogen-activated protein kinase (MAPK) (Cell Signaling Technology), anti-NF-κB p65, anti-p38 MAPK, anti-phospho-extracellular signal-regulated kinase (ERK), and anti-ERK2 from Santa Cruz Biotechnology; and anti-collagen type I cleavage site from ImmunoGlobe. Anti-MMP-1 and procollagen α1(I) N-propeptide (pN-ColIα1) antibodies have been previously described [21 (link)]. Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG and goat anti-rabbit IgG were purchased from KOMA Biotech (Seoul, Korea). Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) and rhodamine Red-X-conjugated goat anti-mouse IgG were purchased from Thermo Fisher Scientific (Waltham, MA, USA). SPlink HRP Detection Bulk Kit for Mouse and Rabbit Antibodies and Liquid AEC Substrate Kit (20×) for IHC were purchased from GBI labs (Bothell, WA, USA).