Three different RDT brands were used in the study. Two products, CareStart™ Malaria Pf (HRP2) Ag RDT and CareStart™ Pf/Pv (HRP2/pLDH) were from the same manufacturer; (AccessBio Inc, Somerset, New Jersey, USA) and the other, CareUs™ Malaria Combo Pf/PAN (HRP2/pLDH) Ag from a different manufacturer (CareUS, Seoul, South Korea). Antigen specificity for the tests were HRP2 (P. falciparum only), HRP2/Pv-LDH (P. falciparum/P. vivax) and HRP2/Pan-LDH (P. falciparum/pan-Plasmodium). All three RDTs met WHO recommended procurement criteria as determined by the WHO RDT product evaluation [23 ].
Carestart malaria pf hrp2 ag rdt
Manufactured by Access Bio
Sourced in United States
The CareStart™ Malaria Pf (HRP2) Ag RDT is a rapid diagnostic test designed to detect the presence of the Plasmodium falciparum (Pf) antigen in human blood samples. The test utilizes an immunochromatographic assay to provide a qualitative result, indicating the presence or absence of the Pf antigen.
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Lab products found in correlation
3 protocols using carestart malaria pf hrp2 ag rdt
1
Comparative Evaluation of Malaria RDTs
2
Rapid Malaria Diagnosis Using CareStart™
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The CareStart™ Malaria Pf (HRP2) Ag RDT (AccessBio, USA) kit was chosen for the present study since it passed WHO-recommended criteria for routine diagnosis [37 ]. Briefly, the RDT was labelled with a unique participant code and date before 5µL of whole blood was pipetted into the sample well, “S”, on the RDT cassette. Afterwards, two drops (60µL) of buffer solution provided by the manufacturer were added to the well labelled “A” on the cassette. Test results were recorded after twenty (20) minutes according to the manufacturer’s instructions.
3
CareStart Malaria Pf HRP2 Ag RDT Protocol
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The CareStart™ Malaria Pf (HRP2) Ag RDT (Access Bio, Inc., Somerset, New Jersey, USA) was used for the qualitative detection of malaria histidine-rich protein 2 in the whole blood according to the manufacturer’s instructions [ACCESSBIO, 2018, Somerset, New Jersey, USA]).
The test membrane strip is pre-coated with a P. falciparum HRP2 specific monoclonal antibody as a single line across the test strip. The reported panel detection score is 91.0% at 200 parasites/µl with a false positive rate of 0.9% [36 , 37 (link)]
The test membrane strip is pre-coated with a P. falciparum HRP2 specific monoclonal antibody as a single line across the test strip. The reported panel detection score is 91.0% at 200 parasites/µl with a false positive rate of 0.9% [36 , 37 (link)]
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