Plasma levels of TF (range: 7.8–500 pg/mL), TFPI (range 31.3–2000 pg/mL) and TM (range 62.5–4000 pg/mL) were measured using ELISA kits, in accordance with the manufacturer’s instructions: TF (cat. DCF300, R&D Systems); TFPI (cat. DTFP10, R&D Systems) and TM (cat. DTHBD0, R&D Systems). Standard curves of known concentrations of recombinant human proteins (TF, TFPI and TM) were used to convert optical density (OD) into concentration units (pg/mL). The plates were read in a SpectraMax Paradigm® machine (Molecular Devices, California, USA). Plasma levels of D-dimer were measured using HemosIL D-Dimer HS 500 kit (cat. 0020500100) on an automated coagulation analyzer ACL TOP 300 CTS (Instrumentation Laboratory, Bedford, MA, USA).
Dthbd0
Manufactured by R&D Systems
DTHBD0 is a laboratory product from R&D Systems. It is a biochemical reagent used for research purposes.
Automatically generated - may contain errors
2 protocols using dthbd0
1
Quantification of Hemostatic Biomarkers
2
Quantification of Plasma TAT, HMGB-1 and Cell Culture TM, HMGB-1
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In the present in vivo study, plasma concentrations of TAT and HMGB‐1 were quantified with each of the ELISA kits: a TAT kit from Cloud‐Clone Corp. (SEA831Mu; Houston, TX) and a HMGB‐1 kit from Shino‐Test Corp. (326054329; Kanagawa, Japan).
In the present in vitro study, concentrations of TM in cell culture supernatant and cell lysate were evaluated by a TM ELISA kit that was purchased from R&D Systems (DTHBD0; Minneapolis, MN). HMGB‐1 levels in cell culture supernatant were quantified by a HMGB‐1 ELISA kit that was purchased from Shino‐Test Corp. (326054329).
In the present in vitro study, concentrations of TM in cell culture supernatant and cell lysate were evaluated by a TM ELISA kit that was purchased from R&D Systems (DTHBD0; Minneapolis, MN). HMGB‐1 levels in cell culture supernatant were quantified by a HMGB‐1 ELISA kit that was purchased from Shino‐Test Corp. (326054329).
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