The proliferation of CDFs or NIH3T3 was evaluated using a 5-Bromo-2′-deoxyuridine (BrdU) incorporation assay kit (Cell Signaling Technology, MA, USA) following the manufacturer's instructions. In brief, CDFs or NIH3T3 were seeded into a 6-well plate with 1 × 106 cells/well and they were treated with different interventions for 24h. Then, BrdU solution was added into each well for another 12 h after interventions. Next, cells were fixed and washed with PBS and incubated with mouse anti-BrdU-primary antibody for 1h at RT. Finally, DAPI was used to counterstain the nucleus for 3 min. Cell proliferation was measured using the average number of BrdU + cells/number of DAPI per mm2.
5 bromo 2 deoxyuridine brdu incorporation assay kit
Manufactured by Cell Signaling Technology
Sourced in United States
The 5-Bromo-2'-deoxyuridine (BrdU) incorporation assay kit is a laboratory tool used to measure cell proliferation. It detects the incorporation of BrdU, a synthetic nucleoside analog, into the DNA of dividing cells during the S phase of the cell cycle.
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2 protocols using 5 bromo 2 deoxyuridine brdu incorporation assay kit
1
BrdU Proliferation Assay for CDFs and NIH3T3
2
Cell Proliferation and Viability Assay
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Cell proliferation was detected by 5-Bromo-2′-deoxyuridine (Brdu) incorporation assay kit (Cell Signaling Technology, USA). 24 h following interventions, cells treated with Brdu agent for another 12 h. Then cells were fixed, washed, and incubated with anti-Brdu antibody for 60 min. Dapi was applied to stain nucleus. Cell proliferation was calculated using the number of Brdu + cells/number of Dapi. Cell viability was assessed by Cell counting kit-8 (CCK-8, Beyotime, China) [28 (link)].
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