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Anti phospho erk1 2 44 680g

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The Anti-phospho ERK1/2 (44-680G) is a laboratory equipment product that can be used to detect and quantify phosphorylated forms of the extracellular signal-regulated kinase 1 and 2 (ERK1/2) proteins. It is designed for use in immunoassays and other research applications.

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Anti erk1 2 44 654g, by Thermo Fisher Scientific (2 mentions)

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ERK1/2 (15 citations) Anti-ERK1/2 (9 citations) Phospho-ERK1/2 (3 citations)

2 protocols using anti phospho erk1 2 44 680g

1

Jurkat T Cell Stimulation Assay

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Jurkat cells: 2x106 Jurkat T cells were stimulated in RPMI at 37°C either CXCL12 (0.2 μM) or anti-CD3ε antibody (1 µg/ml). Reactions were stopped by adding ice-cold PBS and cells were spun down and lysed in RIPA buffer containing protease inhibitors. Samples were analyzed by standard immunoblotting techniques using anti-phospho ERK1/2 (44-680G, Life Technologies) and anti-ERK1/2 (44-654G, Life Technologies) as a loading control.
Köchl R., Thelen F., Vanes L., Brazao T.F., Fountain K., Xie J., Huang C.L., Lyck R., Stein J.V, & Tybulewicz V.L. (2016). WNK1 kinase balances T cell adhesion versus migration in vivo. Nature immunology, 17(9), 1075-1083.
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2

Jurkat T Cell Stimulation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Jurkat cells: 2x106 Jurkat T cells were stimulated in RPMI at 37°C either CXCL12 (0.2 μM) or anti-CD3ε antibody (1 µg/ml). Reactions were stopped by adding ice-cold PBS and cells were spun down and lysed in RIPA buffer containing protease inhibitors. Samples were analyzed by standard immunoblotting techniques using anti-phospho ERK1/2 (44-680G, Life Technologies) and anti-ERK1/2 (44-654G, Life Technologies) as a loading control.
Köchl R., Thelen F., Vanes L., Brazao T.F., Fountain K., Xie J., Huang C.L., Lyck R., Stein J.V, & Tybulewicz V.L. (2016). WNK1 kinase balances T cell adhesion versus migration in vivo. Nature immunology, 17(9), 1075-1083.
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