Anti histone h3k4me3q5ser

Manufactured by Merck Group

Anti-Histone H3K4me3Q5ser is a laboratory reagent that specifically binds to the trimethylated lysine 4 and phosphorylated serine 5 residues on histone H3 proteins. It is commonly used in various applications, such as chromatin immunoprecipitation (ChIP) and Western blotting, to study epigenetic modifications and chromatin dynamics.

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Lab products found in correlation

Ham s f12 medium, by Thermo Fisher Scientific (2 mentions) Anti flag tag, by Affinity Biosciences (2 mentions) Anti histone h3, by Immunoway (2 mentions) Ab178410, by Abcam (1 mentions) Anti lamin a c, by Abcam (1 mentions) Anti tgm2, by Santa Cruz Biotechnology (1 mentions) Anti histone h3k4me3, by Abcam (1 mentions) Anti β actin, by Merck Group (1 mentions) Eagle s minimum essential medium, by American Type Culture Collection (1 mentions) Anti wdr5, by Abcam (1 mentions) Anti actin, by Merck Group (1 mentions) Anti tublin, by Yeasen (1 mentions)

2 protocols using anti histone h3k4me3q5ser

Neuroblastoma Cell Line SK-N-SH Culture

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The human neuroblastoma cell line SK-N-SH was obtained from the Cell Bank of Chinese Academy of Sciences. The SK-N-SH cells were cultured in 1:1 Eagle’s minimum essential medium (American Type Culture Collection) and Ham’s F12 medium (Thermo Fisher Scientific) supplemented with 15% fetal bovine serum and incubated at 37°C in a humidified incubator with 5% CO₂.
The antibodies used in current study included anti-WDR5 (Abcam, ab178410), anti-Histone H3K4me3 (Abcam, ab213224), anti-Histone H3Q5ser (Millipore, ABE1791), anti-Histone H3K4me3Q5ser (Millipore, ABE2580), anti-FLAG tag (Affinity, T0003), anti-Histone H3 (ImmunoWay, YM3038), anti-Lamin A+C (Abcam, ab108595), anti-TGM2 (Santa Cruz Biotechnology, sc-48387), anti–β-actin (Sigma-Aldrich, A3854), horseradish peroxidase (HRP)–conjugated anti-rabbit IgG (CST, 7074S), and HRP-conjugated anti-mouse IgG (CST, 7076S).

Zhao J., Chen W., Pan Y., Zhang Y., Sun H., Wang H., Yang F., Liu Y., Shen N., Zhang X., Mo X, & Zang J. (2021). Structural insights into the recognition of histone H3Q5 serotonylation by WDR5. Science Advances, 7(25), eabf4291.

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Characterization of Human Cell Lines

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The human neuroblastoma cell line SK-N-SH and the human embryonic kidney cell line (HEK 293T) were obtained from the Cell Bank of Chinese Academy of Sciences. The SK-N-SH and HEK 293T cells were cultured in 1:1 Eagle's Minimum Essential Medium (EMEM) (ATCC) and Ham's F12 medium (Thermo) supplemented with 15% fetal bovine serum (FBS) and Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% FBS, and incubated at 37°C in a humidified incubator with 5% CO2.
The antibodies used in current study included anti-WDR5 (Abcam, ab178410); anti-Histone H3K4me3Q5ser (Millipore, ABE2580); anti-FLAG tag (Affinity, T0003); anti-Histone H3 (Immunoway, YM3038); anti--Actin (Sigma, A3854); anti--Tublin (Yeasen, 30303ES50); HRP-conjugated anti-rabbit IgG (CST, 7074S); and HRP-conjugated antimouse IgG (CST, 7076S).

Zhao J., Chen W., Zhang Y., Yang F., Shen N., Zhang X., Mo X., & Zang J. (2020). Structural insights into the recognition of histone H3Q5 serotonylation by WDR5.

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