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Facsdiva data acquisition software

Manufactured by BD
Sourced in United States

FACSDiVa is a data acquisition software for flow cytometry applications. It provides functionality for capturing and recording data generated by flow cytometry instruments. The software enables users to configure and control the instrument, as well as acquire and manage flow cytometry data.

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2 protocols using facsdiva data acquisition software

1

Characterization of Dental Pulp Stem Cells

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The isolated cells were cultured on 100 mm cell culture dishes until 60% confluence. We analyzed the cell surface antigen expression according to previously described methods [22 (link)]. Cells were resuspended in flow cytometry staining buffer (phosphate-buffered saline (PBS) + 2% FBS) at a concentration of 1 × 106 cells/tube, followed by staining with primary specific antibodies, including phycoerythrin-conjugated CD146, APC-conjugated CD90, APC-conjugated CD73, APC-conjugated CD105, APC-conjugated CD34, APC-conjugated CD45, APC-conjugated CD14, and APC-conjugated HLA-DR (eBioscience, San Diego, CA, USA), and incubating for 30 min at 4 °C. Subclass-matched antibodies were used as negative control. The cells were then washed twice and resuspended in 400 µL of 2% FBS/PBS. Flow cytometry was performed using SH800 cell sorter (Sony, Tokyo, Japan); all the data were analyzed using FACSDiVa data acquisition software (BD Biosciences, San Jose, CA, USA). We confirmed that the isolated cells expressed mesenchymal stem cell markers, and not hematopoietic stem cell markers, and defined the isolated cells as DPSCs.
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2

Flow Cytometry Data Analysis

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All data was acquired using FACSDiVa data acquisition software (BD Biosciences) and analyzed using FlowJo version 7.6.5 for PC (FlowJo LLC). Degree of fluorochrome fluorescence was expressed as a staining index (SI), using the median and slope distributions of labeled and background cell or bead populations to calculate a value proportional to the level of fluorochrome fluorescence, as described previously [18 (link)]. Compensation was calculated in FlowJo using the package’s automated spillover matrix algorithm.
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