Palmitic acid p0500

Bovine serum albumin (BSA), essentially fatty acid free (A-7030), and palmitic acid (P-0500) were obtained from Sigma (St. Louis, MO, USA). ¹⁴C-Palmitic acid (850 mCi mmol^-1) (uniformly labeled), dissolved in ethanol, was obtained from Amersham. ¹³¹I-Human serum albumin (750 mCi mmol^-1), dissolved in 0.1 mmol l^-1 phosphate buffer (pH 7.4), manufactured by Squibb, Inc., was obtained through Northwest Radio-pharmaceutical Services, Inc. All other chemicals were of reagent grade and purchased from Baker Chemical Co.
Dialysis bags were made from Spectra/por dialysis membrane (molecular weight cutoff: 12000–14000). The 0.2 μm membrane filters (mixed esters of cellulose acetate and cellulose nitrate) were obtained from Nucleopore, and 8.5 μm membrane filters (mixed esters of cellulose acetate and cellulose nitrate) from Millipore.

Rabbit polyclonal anti MIGA2 antibody (ab 122713) was purchased from Abcam. Rabbit monoclonal anti β-actin antibody was from Cell Signaling Technology (4970; RRID:AB 2223172). Almost all lipids were purchased from Avanti Polar Lipids: DOPC (850375), liver PE (840026), DGS-NTA (Ni; 709404), Liss Rhod PE (810150), Brain PI (4,5) P2 (840046), NBD-PA (810176), NBD-PS (810195), 18:1 NBD-PS (810198), NBD-PC (810133), NBD-PE (810156), NBD-cholesterol (810250), NBD-sphingomylein (810219), 16:0 PA (830855), triolein (18:1 TG; 870110). Palmitic acid (P0500) was ordered from Sigma-Aldrich. Sodium dithionite was from Sigma-Aldrich (157953). Etomoxir was purchased from MedChemExpress (HY-50202). The Hela cell line (ATCC #CCL-2; RRID: CVCL 0030) was a gift from Mals Mariappan (Yale University, New Haven, CT), C. elegans cDNA was a gift from Daniel Colon-Ramos (Yale University, New Haven, CT; Xuan et al., 2017 (link)). Plasmid for the 6xhis-PH-tethering construct (Bian et al., 2018 (link)) was a gift from Pietro De Camilli (Yale University, New Haven, CT).