Antibodies to cyclin F (D9K2U), USP7 (D17C6), UBR5 (D6O8z), LC3 (4775S), and β-Actin (13E5) were from Cell Signaling Technology (MA, USA). Anti-HA-probe (F7) was from Santa Cruz (TX, USA), anti-cyclin A2 (BF683) from Bethyl Laboratories (TX, USA), anti-p53 (DO7) from Abcam (Cambridge, UK), anti-rabbit and anti-mouse IgG-HRP from Genei Laboratories (Bangalore, India), and anti-rabbit IgG-HRP from Cloud-Clone Corp (TX, USA).
Anti p53 do7
Manufactured by Abcam
Sourced in United States
Anti-p53 (DO7) is a primary antibody that recognizes the p53 protein. p53 is a tumor suppressor protein that plays a crucial role in cell cycle regulation and apoptosis. The Anti-p53 (DO7) antibody can be used in various immunoassays, such as Western blotting, immunohistochemistry, and immunocytochemistry, to detect and study the expression of p53 in biological samples.
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Lab products found in correlation
2 protocols using anti p53 do7
1
Antibody Detection Protocol for Cell Signaling
2
Evaluating p53 Stability under Stress Conditions
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For p53 stability experiments, identically transfected cells were treated with 100 μg/ml cycloheximide at ∼48 h posttransfection. Cells were harvested at indicated time points (Figures 1 , 4 , and 5 ) and lysed in RIPA buffer (150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris-HCl, pH 7.4) supplemented with protease inhibitors. Ectopic p53 or endogenous p53 proteins were analyzed by anti-Myc or anti-p53 (DO-7; Abcam, Cambridge, MA) antibody, respectively. To ensure equal loading, we used the stable protein actin as the loading control. For induction of genomic stress, cells were UV irradiated at 25 J/m2. After a 6-h recovery, p53 stability assays were performed as described above.
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