Iq5 gene expression analysis

Manufactured by Bio-Rad

The Bio-Rad IQ5 is a real-time PCR detection system designed for gene expression analysis. It provides precise and reliable quantification of nucleic acid targets. The system is equipped with a thermal cycler and optical detection module to enable accurate and reproducible real-time PCR experiments.

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Lab products found in correlation

Myiq real time pcr system, by Bio-Rad (2 mentions) Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions) Sybr green, by Bio-Rad (1 mentions) Maxima first strand cdna synthesis kit for rt qpcr with dsdnase, by Thermo Fisher Scientific (1 mentions) Maxima sybr green fluorescein qpcr master mix, by Thermo Fisher Scientific (1 mentions)

2 protocols using iq5 gene expression analysis

Quantitative PCR Analysis of Gene Expression

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RNA was reverse transcribed using oligo(dT) primes and RevertAid First Strand cDNA Synthesis Kit (Fermentas).
Quantitative PCR was conducted using Sybr Green and the MyIQ real time PCR system (Bio-Rad). Primers were validated for correct PCR efficiency and are listed in Supplemental Table 1. qPCR samples were analyzed in duplicate, and the relative amounts of each transcript were determined using the ddCT method and the Bio-rad IQ5 gene expression analysis (Bio-Rad). All qPCR expression levels were normalized to levels of histone H4 (Bonnick et al. 2012 (link)).

Holmes G., Herdegen S., Schuon J., Cyriac A., Lass J., Conte C., Calin-Jageman I.E, & Calin-Jageman R.J. (2015). Transcriptional analysis of a whole-body form of long-term habituation in Aplysia californica. Learning & Memory, 22(1), 11-23.

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Quantitative PCR Protocol for Gene Expression

Cited 1 time

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RNA was reverse transcribed using the Maxima First Strand cDNA Synthesis Kit for RT-qPCR with dsDNase (Thermo Scientific, Waltham, MA).
Quantitative PCR was conducted using Maxima SYBR Green/Fluorescein qPCR Master Mix (Thermo Scientific, Waltham, MA)and the MyIQ real time PCR system (Bio-Rad, Los Angeles CA). Primers were validated for correct PCR efficiency and are listed in S1 Table. qPCR samples were analyzed in duplicate and the relative amounts of each transcript were determined using the ddCT method and the Bio-rad IQ5 gene expression analysis (Bio-Rad, Los Angeles CA). All qPCR expression levels were normalized to levels of histone H4, a transcript which is stable during long-term sensitization training [59] (link).

Herdegen S., Conte C., Kamal S., Calin-Jageman R.J, & Calin-Jageman I.E. (2014). Immediate and Persistent Transcriptional Correlates of Long-Term Sensitization Training at Different CNS Loci in Aplysia californica. PLoS ONE, 9(12), e114481.

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