Cholesterol (Fujifilm Wako Pure Chemical Corp., Osaka, Japan), squalene (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), Cholesterol myristate (Tokyo Chemical Industry Co.), Cholesterol palmitate (Tokyo Chemical Industry Co.), and Cholesterol stearate (Sigma-Aldrich Corp.) served as standards during free Cholesterol and squalene analysis. High-performance liquid chromatography (HPLC; 30A (Nexera X2) series), tandem mass spectrometry (MS/MS; LCMS-8060), and Labsolutions software version 5.86 from Shimadzu Corp. were used for measurements. A calibration curve was prepared from a linear regression equation (using the least-squares method) of the peak area ratios of the calibration curve samples. The sample values were calculated by fitting the peak area ratios of the sample solutions and recovered samples to the calibration curve. If lipids other than Cholesterol myristate, Cholesterol palmitate, and Cholesterol stearate were detected, the measured values were calculated using the calibration curves of those standards, which exhibited adjacent retention times.
Cholesterol stearate
Manufactured by Merck Group
Sourced in Japan
Cholesterol stearate is a lipid compound commonly used in laboratory settings. It functions as a standard reference material for the analysis and quantification of cholesterol levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Trioleylglycerol, by Merck Group (2 mentions)
Squalene, by Tokyo Chemical Industry (1 mentions)
Cholesterol, by Fujifilm (1 mentions)
Squalene, by Merck Group (1 mentions)
Labsolutions software version 5, by Shimadzu (1 mentions)
Lcms 8060, by Shimadzu (1 mentions)
Silica gel 60 plate, by Merck Group (1 mentions)
3 protocols using cholesterol stearate
1
Quantification of Cholesterol and Squalene
2
Yeast Lipid Profiling Protocol
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Yeast strains were grown in synthetic medium (with 2% glucose) to stationary phase. Lipids were isolated from ∼100 OD of cells by the method of Folch et al. (1957) (link) with modifications. Briefly, lipids were extracted with chloroform: methanol (2:1) and washed with 1 M KCl. Lipid extracts were resolved by TLC on silica gel 60 plates (Merck, Darmstadt, Germany) in petroleum ether/diethyl ether/acetic acid (80:20:1) and visualized with methanolic MnCl2, followed by plate charring. Band intensities were quantified using ImageJ software (National Institutes of Health, Bethesda, MD) after digital scanning. Standards included trioleylglycerol, dioleylglycerol, and cholesterol stearate (Sigma-Aldrich).
3
Quantifying Lipid Incorporation Dynamics
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Incorporation of [3H]oleate into TAG and SE species was quantified as previously described (Oelkers et al., 2000 (link)). Log-phase cultures cultured in YPD were pulsed with [3H]oleate (1 μCi/ml) for 30 min at 30ºC. Total lipids were extracted, resolved by TLC, and stained with iodine vapor. TAG and SE species were identified using trioleylglycerol and cholesterol stearate (Sigma-Aldrich) as standards and individually harvested, and radiolabel in each fraction was determined by liquid scintillation counting.
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