Avance 500 ultrashield nmr spectrometer

Manufactured by Bruker

The AVANCE 500) UltraShield-NMR spectrometer is a nuclear magnetic resonance (NMR) spectrometer designed and manufactured by Bruker. It is a high-performance instrument used for the analysis and characterization of chemical compounds and materials. The AVANCE 500) UltraShield-NMR spectrometer provides advanced NMR capabilities for researchers and scientists in various fields, including chemistry, biochemistry, and materials science.

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Lab products found in correlation

Agilent 1290 infinity 2 lc system, by Agilent Technologies (1 mentions)

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Avance Ultrashield NMR spectrometer Avance 500 NMR spectrometer Avance Ultrashield spectrometer Ultrashield NMR spectrometer UltraShield 500 spectrometer Avance NMR spectrometer Avance 500 spectrometer 500 NMR spectrometers Ultrashield 500 Avance 500

3 protocols using avance 500 ultrashield nmr spectrometer

Isolation and Characterization of Organic Compounds

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Analytical-grade solvents, including hexane and dichloromethane used for extraction and isolation, were purchased from (Merck KGaA, Darmstadt, Germany). Column chromatography was carried out using silica gel Merck 7734 (70–230 mesh ASTM) and Merck 9385 (230–400 mesh ASTM). Thin-layer chromatography (TLC) analyses were carried out on Merck silica gel DC-plastikfolien 60 F254 plastic sheets and TLC spots were visualized using a UV lamp at 254 and 366 nm, respectively. Melting point was recorded using an electrothermal digital melting point apparatus. Ultraviolet (UV) and IR spectra were recorded on a Varian UV–visible light (UV–Vis) 50 and Perkin-Elmer 100 Fourier-transform infrared (FTIR) spectrophotometers, respectively. The isolated compound was dissolved in CDCl₃ (deuterated chloroform), and the NMR spectra (1D and 2D NMR) were recorded on a Bruker AVANCE 500 Ultrashield NMR spectrometer. The molecular weight of the isolated compound was determined on an Agilent 1290 Infinity II LC system (Agilent Technologies, Santa Carla, CA, USA) coupled to a 6520 Q-TOF tandem mass spectrometer for separation. The chemical shifts (δ) were determined from the residual solvent peaks.

Karunanidhi A., Ghaznavi-Rad E., Jeevajothi Nathan J., Joseph N., Chigurupati S., Mohd Fauzi F., Pichika M.R., Hamat R.A., Lung L.T., van Belkum A, & Neela V. (2019). Bioactive 2-(Methyldithio)Pyridine-3-Carbonitrile from Persian Shallot (Allium stipitatum Regel.) Exerts Broad-Spectrum Antimicrobial Activity. Molecules, 24(6), 1003.

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NMR Characterization of Solute in D2O

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SG was dissolved in deuterium oxide (D₂O), and ¹H and ¹³C nuclear magnetic resonance spectra were acquired on a Bruker (AVANCE 500) UltraShield-NMR spectrometer at 80°C. Sodium 2,2-dimethylsilapentane-5-sulphonate (DSS) was used as the internal standard (0.00 ppm for ¹H). ¹H and ¹³C NMR chemical shifts were measured in ppm relative to internal reference D₂O at 4.7 ppm. A distortion-less enhancement by polarization transfer (DEPT 135) spectrum was recorded to determine each carbon’s hydrogenation; the acquisition and delay times were 1.0 s. 2D ¹H and ¹³C Heteronuclear Single Quantum Coherence (HSQC) spectra were acquired using the pulse programs supplied with the apparatus.

Krueger A., Baumann S., Krammer P.H, & Kirchhoff S. (2001). FLICE-Inhibitory Proteins: Regulators of Death Receptor-Mediated Apoptosis. Molecular and Cellular Biology, 21(24), 8247-8254.

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NMR Spectroscopy of Deuterated Compound

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SG was dissolved in deuterium oxide (D 2 O), and 1 H and 13 C nuclear magnetic resonance spectra were acquired on a Bruker (AVANCE 500) UltraShield-NMR spectrometer at 80°C. Sodium 2,2-dimethylsilapentane-5sulphonate (DSS) was used as the internal standard (0.00 ppm for 1 H). 1 H and 13 C NMR chemical shifts were measured in ppm relative to internal reference D 2 O at 4.7 ppm. A distortion-less enhancement by polarization transfer (DEPT 135) spectrum was recorded to determine each carbon's hydrogenation; the acquisition and delay times were 1.0 s. 2D 1 H and 13 C Heteronuclear Single Quantum Coherence (HSQC) spectra were acquired using the pulse programs supplied with the apparatus.

Khongthong S., Theapparat Y., Roekngam N., Tantisuwanno C., Otto M, & Piewngam P. (2021). Characterization and immunomodulatory activity of sulfated galactan from the red seaweed Gracilaria fisheri. International journal of biological macromolecules, 189.

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