Trimethylpyruvic acid

Manufactured by Merck Group

Sourced in United States

Trimethylpyruvic acid is a chemical compound that serves as an analytical standard and research reagent. It has the molecular formula C₆H₁₀O₃. The compound is used in various chemical and biochemical applications, including analysis and research, but a detailed description of its core function is not available without the risk of interpretation or extrapolation.

Automatically generated - may contain errors

Lab products found in correlation

Primerstar max dna polymerase, by Takara Bio (2 mentions) E coli bl21 de3 competent cell, by Transgene (2 mentions) Sds page kit, by Transgene (2 mentions) L tert leucine, by Merck Group (2 mentions) D tert leucine, by Merck Group (2 mentions) Lb broth, by Sangon (2 mentions) D glucose, by Sangon (2 mentions) Modified bradford protein assay kit, by Sangon (1 mentions) Pcr purification kit, by Omega Bio-Tek (1 mentions)

2 protocols using trimethylpyruvic acid

Expression and Purification of Recombinant Proteins

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Primer STAR Max DNA Polymerase, restriction enzymes, ligation enzyme were purchased from Takara (Shiga, Japan). pET-28a (+) expression vector was provided from Novagen. Plasmid, gel extraction, and PCR purification kits were provided from Omega bio-tek(USA). E. coli BL21 (DE3) competent cell, Isopropyl-β-d-thiogalactopyranoside (IPTG), kanamycin and SDS-PAGE kit were purchased by Transgen (Beijing, China). Modified Bradford Protein Assay Kit, LB broth, d-glucose, NADH and NAD⁺ were purchased by Sangon biotech (Shanghai, China). Trimethylpyruvic acid, l-tert-leucine and D-tert-leucine were bought from Sigma-Aldrich (USA).

Liao L., Zhang Y., Wang Y., Fu Y., Zhang A., Qiu R., Yang S, & Fang B. (2021). Construction and characterization of a novel glucose dehydrogenase-leucine dehydrogenase fusion enzyme for the biosynthesis of l-tert-leucine. Microbial Cell Factories, 20, 3.

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Recombinant Protein Expression and Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primer STAR Max DNA Polymerase, restriction enzymes, ligation enzyme were purchased from Takara (Shiga, Japan). pET-28a (+) expression vector was provided from Novagen. Plasmid, gel extraction, and PCR puri cation kits were provided from Omega bio-tek(USA). E. coli BL21 (DE3) competent cell, Isopropyl-β-D-thiogalactopyranoside (IPTG), kanamycin and SDS-PAGE kit were purchased by Transgen (Beijing, China). Modi ed Bradford Protein Assay Kit, LB broth, D-glucose, NADH and NAD + were purchased by Sangon biotech (Shanghai, China). Trimethylpyruvic acid, L-tert-leucine and D-tert-leucine were bought from Sigma-Aldrich (USA).

Liao L., Zhang Y., Wang Y., Fu Y., Zhang A., Qiu R., Yang S., & Fang B. (2020). Construction and Characterization of a Novel Glucose Dehydrogenase-Leucine Dehydrogenase Bifunctional Enzyme for the Biosynthesis of L-Tert-Leucine.

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