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2 protocols using 4 2 hydroxyethyl piperazine 1 ethanesulfonic acid sodium salt

1

Formulation and Evaluation of Berberine-Loaded Lipid Carriers

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Berberine chloride (BER), amphotericin B (AmB), dimethyl sulfoxide (DMSO), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt (HEPES), adenine, hemin, biotin, ethylenediaminetetraacetic acid (EDTA), cholesterol (Chol), dicetyl phosphate (DP), didodecyldimethylammonium bromide (DDAB), d-α-tocopherol succinate (TS) and d-(+)-trehalose dihydrate were obtained from Sigma (St. Louis, MO, USA). Soybean phosphatidylcholine (PC) was kindly gifted by Lipoid GmbH (Ludwigshafen, Germany). Fungizone® (FGZ) was obtained from X-GEN Pharmaceuticals, Inc. (Horseheads, NY, USA). All other reagents were of analytical grade.
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2

Synthesis and Characterization of Gold Nanoparticles

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Tetrachloroauric(III) acid (HAuCl 4 •3H 2 O, 99.9%), cetyltrimethylammonium bromide (CTAB, 99%), sodium borohydride (NaBH 4 , 99.99%), silver nitrate (AgNO 3 , 99.99%), L-ascorbic acid, hydrochloric acid (37%), poly(diallyldimethylammonium chloride) (PDADMAC, MW ∼ 200 000-350 000, 20 wt% in H 2 O), polystyrene sodium sulfonate (PSS, MW ∼ 70 000), poly(vinyl alcohol) (PVA, MW ∼ 124 000), 4-(2-hydroxyethyl) piperazine-1ethanesulfonic acid sodium salt (HEPES, 99.5%), n-dodecyl β-D maltoside (β-DM, MW ∼ 50 000) were purchased from Sigma-Aldrich. All chemicals were used as received without any further purification. Milli-Q (18 MΩ cm at 27 °C) water was used in the preparation of all solutions.
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