Cd4 fitc sfci12t4d11

Manufactured by Beckman Coulter

Sourced in United States

CD4-FITC (SFCI12T4D11) is a fluorescently labeled monoclonal antibody used for the identification and enumeration of CD4+ T cells by flow cytometry. It binds specifically to the CD4 antigen expressed on the surface of human T helper cells.

Automatically generated - may contain errors

Lab products found in correlation

Lsr 2 cytometer, by BD (2 mentions) Cd326 bv650, by BioLegend (2 mentions) Cd19 percp cy5, by BioLegend (2 mentions) Cd45ra apc efluor780 hi100, by Thermo Fisher Scientific (2 mentions) Cd8 percp cy5, by BioLegend (2 mentions) Cd3 pacific blue, by BD (2 mentions) Cd4 alexafluor700, by BD (1 mentions) Cd66b percp cy5.5 g10f5, by BioLegend (1 mentions) Ccr7 fitc 150503, by R&D Systems (1 mentions) Hiv p24 rd1 fh190 1 1, by Beckman Coulter (1 mentions) Cd3 alexa700, by BioLegend (1 mentions) Hla dr bv785, by BioLegend (1 mentions) Cd3 alexa700, by Thermo Fisher Scientific (1 mentions) Cd4 alexa fluor 700 rpa t4, by BD (1 mentions) Cd66b percp cy5, by BioLegend (1 mentions) Liberase dl, by Roche (1 mentions)

Close spelling variants of this product

CD4-FITC (17 citations) SFCI12T4D11 (2 citations)

2 protocols using cd4 fitc sfci12t4d11

Polychromatic Flow Cytometry of Immune Markers

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following fluorochrome-conjugated antibodies (Abs) were used for polychromatic flow cytometry analysis: CD3-Pacific-blue (UCHT1), CD4-Alexa-Fluor 700 (RPA-T4), CCR4-PE-Cy7 (1G1), CCR6-PE (11A9), CCR7-PE-Cy7 (3D12), CXCR3-PE-Cy5 (1C6) (BD Biosciences, San Diego, California, USA), CCR7-FITC (150503) (R&D Systems, Minneapolis, Minnesota, USA), CD45RA-APC-eFluor780 (HI100) (eBioscience, San Diego, California, USA), CD3-Alexa700 (UCHT1), CD326-BV650 (9C4), CD8-PerCP-Cy5.5 (RPA-T8), CD19-PerCP-Cy5.5 (HIB19), CD66b-PerCP-Cy5.5 (G10F5), human leukocyte antigen - antigen D related (HLA-DR)-BV785 (L243; Biolegend ,San Diego, California USA), CD4-FITC (SFCI12T4D11), and HIV-p24-RD1 (FH190–1–1); (Beckman Coulter, Fullerton, California, USA). Live/Dead Fixable Aqua Dead Cell Stain Kit (Vivid; Life Technologies, Burlington, Ontario, Canada) was used to exclude dead cells. Cells were analyzed by fluorescence-activated cell sorting (FACS) using the BD-LSRII cytometer and BD-Diva (BD Biosciences) and FlowJo (Tree Star, Inc., Ashland, Oregon, USA) softwares. All Abs were titrated for an optimal noise/signal ratio and Ab cocktails were validated by comparing single with multiple staining. Positive gates were placed based on fluorescence minus one controls [41 (link),69 ].

Gosselin A., Wiche Salinas T.R., Planas D., Wacleche V.S., Zhang Y., Fromentin R., Chomont N., Cohen É.A., Shacklett B., Mehraj V., Ghali M.P., Routy J.P, & Ancuta P. (2016). HIV persists in CCR6+CD4+ T cells from colon and blood during antiretroviral therapy. AIDS (London, England), 31(1), 35-48.

+ Open protocol

+ Expand

Immune Cell Profiling in Sigmoid Colon

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sigmoid colon biopsies (SCB) were processed using Liberase DL (Roche Diagnostics). Matched blood was collected and PBMCs)were isolated by gradient density centrifugation. Cells were stained with the following fluorochrome-conjugated antibodies: CD3-Pacific-Blue (UCHT1), CD4-Alexa-Fluor 700 (RPA-T4; BD Biosciences), CD45RA-APC-eFluor780 (HI100) (eBioscience), CD3-Alexa700 (UCHT1), CD326-BV650 (9C4), CD8-PerCP-Cy5.5 (RPA-T8), CD19-PerCP-Cy5.5 (HIB19), CD66b-PerCP-Cy5.5 (G10F5; Biolegend), CD4-FITC (SFCI12T4D11; Beckman Coulter), CD64-APC (10.1.1; Miltenyi). Aqua Vivid Dead Cell Stain Kit (Life Technologies) was used to exclude dead cells. Phenotypic analysis was performed by flow cytometry using BD-LSRII cytometer, BD-Diva (BD Biosciences) and FlowJo software (Tree Star). Detailed sample processing informations are included in our previous publication [70 (link)].

Gabriel E.M., Wiche Salinas T.R., Gosselin A., Larouche-Anctil E., Durand M., Landay A.L., El-Far M., Tremblay C.L., Routy J.P, & Ancuta P. (2021). Overt IL-32 Isoform Expression at Intestinal Level during HIV-1 Infection is Negatively Regulated by IL-17A. AIDS (London, England), 35(12), 1881-1894.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!