Anti cd4 percp cy5.5 clone rm4 5

Manufactured by BD

Anti-CD4 PerCP-Cy5.5 (clone RM4-5) is a fluorochrome-conjugated antibody used for flow cytometric analysis of CD4+ T cells. It binds to the CD4 cell surface antigen, which is expressed on a subset of T lymphocytes.

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Lab products found in correlation

Foxp3 staining kit, by Thermo Fisher Scientific (1 mentions) Anti cd44 apc cy7 clone im7, by BD (1 mentions) Ki67 pe clone b56, by BD (1 mentions) Fortessa flow cytometer, by Tree Star (1 mentions) Accuri c6 flow cytometer, by BD (1 mentions) Anti cd45 pe cy7, by BioLegend (1 mentions) Anti ly6c apc clone al 21, by BD (1 mentions)

3 protocols using anti cd4 percp cy5.5 clone rm4 5

Multi-parameter flow cytometry analysis of T cells

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Blood was collected by retro-orbital puncture. Spleen cells were obtained by mechanical disruption. After Fc receptor blocking (clone 2.4G2, 1:1,000; BD Biosciences), cells were stained with anti-CD4 PerCP-Cy5.5 (clone RM4-5; 1:800; BD Biosciences) or CD4 BV785 (clone RM4-5; 1:800; BioLegend), anti-TCR-β APC (clone H57-597; 1:200; BD Biosciences) or PE (clone H57-597; 1:100; BD Biosciences), anti-NKp46 FITC (clone 29A1.4; 1:300; eBiosciences), anti-CD8 Alexa700 (clone 53-6.7; 1:1,600; BioLegend), anti-CD25 PE-Cy7 (clone PC61; 1:300; eBiosciences), anti-CD62L BV605 (clone MEL-14; 1:800; BioLegend) and anti-CD44 APC-Cy7 (clone IM7; 1:400; BD Biosciences) in PBS/3% FCS. Then anti-Foxp3 eFluor450 (clone FJK-16 s; 1:150; eBioscience) and Ki67-PE (clone B56; 1:50; BD Biosciences) or Ki67-Alexa647 (clone B56; 1:50; BD Biosciences) staining was performed using the eBioscience Foxp3 staining kit according to the manufacturer's instructions. Cells were acquired on a Fortessa flow cytometer and analysed with FlowJo software (TreeStar).

Pérol L., Lindner J.M., Caudana P., Nunez N.G., Baeyens A., Valle A., Sedlik C., Loirat D., Boyer O., Créange A., Cohen J.L., Rogner U.C., Yamanouchi J., Marchant M., Leber X.C., Scharenberg M., Gagnerault M.C., Mallone R., Battaglia M., Santamaria P., Hartemann A., Traggiai E, & Piaggio E. (2016). Loss of immune tolerance to IL-2 in type 1 diabetes. Nature Communications, 7, 13027.

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Phenotypic Analysis of MLN-derived T Cells

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MLN‐ derived T cell populations were phenotypically analyzed as described in Myhill et al.^[¹⁷^] Briefly, MLN cell suspensions were added to a 96 well plate for surface staining with anti‐mouse TCRβ‐FITC (clone H57‐597, BD Biosciences) and anti‐CD4‐PerCP‐Cy5.5 (clone RM4‐5, BD Biosciences). Anti‐T‐bet‐Alexafluor 647 (clone 4B10, BD Biosciences), anti‐GATA3‐PE (clone TWAJ, Thermo Fisher Scientific), and anti‐Foxp3‐FITC (clone FJK‐16s, Thermo Fisher Scientific) were used for intracellular staining. MLN cell analysis was carried out on a BD Accuri C6 flow cytometer, and data acquired using Accuri CFlow Plus software (Accuri Cytometers).

Zhu L., Myhill L.J., Andersen‐Civil A.I., Thamsborg S.M., Blanchard A, & Williams A.R. (2022). Garlic‐Derived Organosulfur Compounds Regulate Metabolic and Immune Pathways in Macrophages and Attenuate Intestinal Inflammation in Mice. Molecular Nutrition & Food Research, 66(7), 2101004.

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Multiparametric FACS Analysis of Immune Cells

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The following antimouse antibodies were used for FACS staining: anti-Ly6C-APC (clone AL-21, at a dilution of 1/100; BD Biosciences), anti NK1.1-Alexa Fluor 700 (clone PK136, 1/50; BD Biosciences), anti-CD4-PercP/Cy5.5 (clone RM4-5, 1/100; BD Biosciences), anti-CD8a-BV605 (clone 53-6.7, 1/200; BD Biosciences), anti-Ly6G-BV711 (clone 1A8, 1/400; BD Biosciences), anti-CD11c-BV786 (clone HL3, 1/100; BD Biosciences), anti-CD90.2-BV480 (clone 53-2.1, 1/200; BD Biosciences), anti-CD3e-BV650 (clone 145-2C11, 1/100; BD Biosciences), anti-CD45R/B220-BUV395 (clone RA3-6B2, 1/100; BD Biosciences), anti-CD11b-BUV737 (clone M1/70, 1/100; BD Biosciences), anti-CD16/CD32 purified (clone 2.4G2, 1/200; BD Biosciences), anti-γδ T-cell receptor-FITC (clone GL3, 1/100; BD Biosciences), anti-F4/80-PE (clone T45-2342, 1/100; BD Biosciences), and anti-CD45-PE/Cy7 (clone I3/2.3, 1/100; BioLegend, Lucerna-Chem).

Goldstein J.D., Bassoy E.Y., Caruso A., Palomo J., Rodriguez E., Lemeille S, & Gabay C. (2020). IL-36 signaling in keratinocytes controls early IL-23 production in psoriasis-like dermatitis. Life Science Alliance, 3(6), e202000688.

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