Hp innowax

Manufactured by Hewlett-Packard

Sourced in United States

HP-Innowax is a laboratory equipment product designed for chromatographic analysis. It is a high-performance stationary phase used in gas chromatography (GC) and liquid chromatography (LC) applications. The core function of HP-Innowax is to provide efficient separation and analysis of a wide range of chemical compounds.

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Lab products found in correlation

Hp 6890, by Hewlett-Packard (7 mentions) Pure standards, by Merck Group (1 mentions) Buffered peptone water (bpw), by Biolife (1 mentions)

9 protocols using hp innowax

Fatty Acid Profiling in Livestock

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Fatty acids in the feed were identified, after extraction and methylation, using a gas chromatograph (Hewlett Packard HP-6890, Palo Alto, CA, USA) with a capillary column (HP-Innowax, 30 m × 0.32 mm i.d., and 0.25 µm polyethylene glycol-film thickness and a flame ionization detector [29 (link)]). Data are detailed in Supplementary File S1.
The lipids from brain, liver, and muscle (intramuscular fat; IMF) were extracted (expressed as a dry matter percentage [30 (link)] and fractionated into the main lipid fractions: neutral lipids (triglycerides) and polar lipids (phospholipids) [31 (link)]). Finally, FAs were methylated and identified [32 (link)]. Fatty acids of subcutaneous back-fat were also separately analyzed in outer and inner layers. From individual FA values, proportions of saturated, monounsaturated, and polyunsaturated FA (SFA, MUFA, and PUFA), the unsaturated index (UI), and the sum of total n−3 FA (∑n−3) and ∑n−6 FA and its ratio (∑n−6/∑n−3) were calculated [33 (link)]. Moreover, the activity of stearoyl-CoA desaturase enzyme 1 (SCD1) was estimated using the desaturation indexes, ratios of C18:1/C18:0 and MUFA/SFA [34 (link)].

Vázquez-Gómez M., Garcia-Contreras C., Pesantez-Pacheco J.L., Torres-Rovira L., Heras-Molina A., Astiz S., Óvilo C., Isabel B, & Gonzalez-Bulnes A. (2020). Differential Effects of Litter Size and Within-Litter Birthweight on Postnatal Traits of Fatty Pigs. Animals : an Open Access Journal from MDPI, 10(5), 870.

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Fatty Acid Profiling from Plasma and Muscle

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Lipids from blood plasma samples and biceps femoris muscle were extracted and methylated using the procedure described by [22 (link)]. Fat extracts were methylated in the presence of sulfuric acid and analyzed by gas chromatography. Previously fatty acid methyl ester (FAME) samples were identified by gas chromatography, as described elsewhere [23 (link)]. GC-MS was performed using an HP-6890 (Hewlett Packard, Avondale, PA, USA) gas chromatograph, equipped with a flame ionization detector and capillary column (HP-Innowax, 30 m by 0.32 mm ID and 0.25 μm polyethylene glycol-film thickness). A temperature program of 170 °C to 245 °C was used. The injector and detector were maintained at 250 °C. The carrier gas (helium) flow rate was 2 mL/min. For the identification of each fatty acid, pure standards were used (Sigma). The concentration of individual fatty acids was calculated as a % of total fatty acids. The results were expressed as grams per 100 g of detected FAMEs.

Fernández J., de la Fuente V.G., García M.T., Sánchez J.G., Redondo B.I., Villar C.J, & Lombó F. (2020). A diet based on cured acorn-fed ham with oleic acid content promotes anti-inflammatory gut microbiota and prevents ulcerative colitis in an animal model. Lipids in Health and Disease, 19, 28.

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Fatty Acid Profile Analysis

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The product’s fatty acid profile was calculated by isolating lipids from samples by chloroform/methanol extraction, as described by the Folch method. Thin-layer chromatography was utilized to examine the extracted lipids for purity. Fatty acid composition was measured using an Agilent Technologies, USA HP-Innowax 30 m × 0.25 mm × 0.25 m column on a Hewlett Packard HP 6890 gas chromatograph with a flame ionization detector. The analysis was performed in triplicate.

Ashakayeva R., Assenova B., Tumenova G., Nurgazezova A., Zhumanova G., Atambayeva Z., Baikadamova A., Il D, & Dautova A. (2022). A Pumpkin-Based Emulsion Gel as a Texture Improvement of Mixed Horsemeat Semi-Smoked Sausages. Foods, 11(23), 3886.

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Fatty Acid Extraction and Methylation

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The one-step procedure proposed by Sukhija and Palmquist (1988) (link) was used for the extraction and methylation of dietary FA. FA methyl esters were identified with a gas chromatograph (HP-6890; Hewlett Packard, Palo, Alto, CA, USA) with a flame ionisation detector and a capillary column (HP-Innowax: 30 m length, 0.32 mm i.d., 0.25 mm polyethylene glycol film thickness) (Lopez-Bote et al. 1997) (link).

Vázquez-Gómez M., García-Contreras C., Astiz S., Torres-Rovira L., Fernández-Moya E., Olivares A., Daza A., Óvilo C., González-Bulnes A., & Isabel B. (2020). Piglet birthweight and sex affect growth performance and fatty acid composition in fatty pigs. Animal Production Science, 60(4), 573-573.

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Quantifying Biceps Femoris Intramuscular Fat

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Biceps femoris muscle IMF content was quantified using the method proposed by Segura and López-Bote [30 (link)] based on gravimetrical determination of lipid content. Fatty acid methyl esters (FAMEs) were identified by gas chromatography as described by López-Bote et al [31 ] using a Hewlett Packard HP-6890 (Avondale, PA, USA) gas chromatograph equipped with a flame ionization detector and a capillary column (HP-Innowax, 30 m × 0.32 mm i.d. and 0.25 μm polyethylene glycol-film thickness). Results were expressed as grams per 100 grams of detected FAMEs.

Ayuso M., Fernández A., Núñez Y., Benítez R., Isabel B., Barragán C., Fernández A.I., Rey A.I., Medrano J.F., Cánovas Á., González-Bulnes A., López-Bote C, & Ovilo C. (2015). Comparative Analysis of Muscle Transcriptome between Pig Genotypes Identifies Genes and Regulatory Mechanisms Associated to Growth, Fatness and Metabolism. PLoS ONE, 10(12), e0145162.

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Intramuscular Fat Content Quantification

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Longissimus dorsi muscle IMF content was quantified using the method proposed by Segura et al. [31 (link)] based on gravimetrical determination of lipid content. Fatty acid methyl esters (FAMEs) were identified by gas chromatography as described by López-Bote et al. [32 ] using a Hewlett Packard HP-6890 (Avondale, PA, USA) gas chromatograph equipped with a flame ionization detector and a capillary column (HP-Innowax, 30 m × 0.32 mm i.d. and 0.25 μm polyethylene glycol-film thickness). Results were expressed as grams per 100 grams of detected FAMEs.

Ayuso M., Fernández A., Núñez Y., Benítez R., Isabel B., Fernández A.I., Rey A.I., González-Bulnes A., Medrano J.F., Cánovas Á., López-Bote C.J, & Óvilo C. (2016). Developmental Stage, Muscle and Genetic Type Modify Muscle Transcriptome in Pigs: Effects on Gene Expression and Regulatory Factors Involved in Growth and Metabolism. PLoS ONE, 11(12), e0167858.

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Analytical GC of Volatile Compounds

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Analytical gas chromatography (GC) was carried out on a Hewlett-Packard 6890 apparatus (Agilent Technologies, Palo Alto, CA, United States) equipped with a flame ionization detector (FID) and an electronic pressure control injector. An HP Innowax (polyethylene glycol) column (Hewlett-Packard; 30 m × 0.25 mm × 0.25 µm film thickness) was used at a carrier gas flow (nitrogen) of 1.6 mL/min. The split ratio was 60:1. The analysis was performed in triplicate using the following temperature program: oven temperature kept isothermally for 10 minutes at 35°C, increased to 205°C at the rate of 3°C/min, and kept isothermally at 205°C for 10 minutes. Detector and injector temperature were held at 300°C and 250°C, respectively.

Jihene A., Essid R., Karoui I.J., Hammami M., Tabbene O., & Abderrabba M. (2020). Antileishmanial Potential of Propolis Essential Oil and Its Synergistic Combination With Amphotericin B. Natural Product Communications, 15(1), 1934578X1989956-1934578X1989956.

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Fatty Acid Profiling of Fat Layers

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Each fat sample was separated into inner layer and outer layer, and each layer was independently analyzed because they may have different metabolic activity [19 ]. Lipids were extracted following the method of Bligh and Dyer [20 (link)]. Fat extracts were methylated in the presence of sulfuric acid and later analyzed using a gas chromatograph (HP-6890, Hewlett Packard Co., Avondale, PA, USA) equipped with a flame ionization detector and a capillary column (HP-Innowax, 30 m length × 0.32 mm id × 0.25 µm cross-linked polyethylene glycol) [21 (link)]. The proportions of total saturated (SFA), monounsaturated (MUFA), and polyunsaturated fatty acids (PUFA), and also PUFA/SFA ratio, total ꞷ-3 and ꞷ-6, and ꞷ-6/ꞷ-3 ratio were calculated from the individual fatty acid proportions.

Pérez-Ciria L., Carcò G., Miana-Mena F.J., Mitjana O., Falceto M.V, & Latorre M.A. (2021). Immunocastration in Gilts: A Preliminary Study of the Effect of the Second Dose Administration Time on Growth, Reproductive Tract Development, and Carcass and Meat Quality. Animals : an Open Access Journal from MDPI, 11(2), 510.

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Determination of Listeria Fatty Acids

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To analyze the total cellular fatty acids, Listeria strains were incubated after irradiation treatment in buffered peptone water (Biolife Italiana) for 24 h at 37 °C. Each experiment was performed in triplicate by using three independently grown cultures. Cells recovered from 10 ml suspension were pretreated following the Microbial Identification Inc (MIDI) protocols [19] . All reagents for saponification, methylation, extraction, and washing were dispensed with autopipets into the same tube, making the hands-on time minimal. Next, the final extracts were analyzed by gas chromatography (column: 30 m × 0.25 mm HP-Innowax; flame ionization detect temperature at 280°C; carrier gas N2 at 1 ml/min; injector temperature 270°C; oven temperature programmed from 130 to 230°C) using a Hewlett-Packard HP 5890 capillary gas chromatograph linked to an HP Chemstation integrator. The identification of fatty acid methyl esters was performed by external standards (all purchased from Sigma Chemical Co) submitted to the same processes of manipulation as the biological samples analyzed. Total saturated fatty acids (SFA), total unsaturated fatty acids (UFA) were used to determine the differences among membrane fatty acids of L. monocytogenes cells, and they were examined under the different conditions. The UFA/SFA ratio was used as an indirect indicator of the membrane fluidity.

Elabed H. (2021). Morphological Alterations and Virulence Gene Expression of Listeria Monocytogenes Cells in Response to Gamma Irradiation Treatments. Nutrition and Food Processing, 04(5), 01-09.

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