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Masson s trichrome staining kit

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Masson's trichrome staining kit is a laboratory product designed to assist in the histological analysis of tissue samples. It is a three-color staining method used to differentiate collagen, muscle, and nuclei within a specimen. The kit provides the necessary reagents and instructions to perform this specialized staining technique.

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Lab products found in correlation

Acetonitrile, by Thermo Fisher Scientific (2 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) Eclipse te2000 s, by Nikon (1 mentions) Dmi4000b fluorescence microscope, by Leica (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Anti cyclin d1, by Cell Signaling Technology (1 mentions) Rock inhibitor y 27632, by MedChemExpress (1 mentions) Anti phosphorylated yap, by Cell Signaling Technology (1 mentions) Anti phosphorylated smad2, by Cell Signaling Technology (1 mentions) Anti gapdh, by Bio-Synthesis (1 mentions) Anti mlc2, by Cell Signaling Technology (1 mentions) Anti β actin, by Bio-Synthesis (1 mentions) Anti rock, by Cell Signaling Technology (1 mentions) Cytochalasin d, by Abcam (1 mentions) Sch79797, by Bio-Techne (1 mentions) Anti smad2, by Cell Signaling Technology (1 mentions) Anti yap, by Santa Cruz Biotechnology (1 mentions) Anti α sma, by Cell Signaling Technology (1 mentions)

10 protocols using masson s trichrome staining kit

1

Histological Analysis of Pulmonary Fibrosis

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Following sacrificing the animals, the lungs were removed and the left lung was fixed with 4% paraformaldehyde for 16 h. The tissue samples were then processed using graded alcohol, xylene and paraffin and blocked in paraffin. The paraffin-embedded sections (5 μm thick) were stained using a hematoxylin and eosin (H&E) kit (Biyuntian, Inc., Nantong, China) and a Masson’s trichrome staining kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), according to the manufacturer’s instructions. The slides with H&E staining were examined using light microscopy (Eclipse TE2000-S; Nikon Corporation, Tokyo, Japan) and images were captured to determine the integrity of the tissue. The severity of pulmonary fibrosis in the lung sections stained for collagen with Masson’s trichrome stain was determined by a histopathologist who was blinded to the protocol design.
YANG Z., SUN Z., LIU H., REN Y., SHAO D., ZHANG W., LIN J., WOLFRAM J., WANG F, & NIE S. (2015). Connective tissue growth factor stimulates the proliferation, migration and differentiation of lung fibroblasts during paraquat-induced pulmonary fibrosis. Molecular Medicine Reports, 12(1), 1091-1097.
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2

Penile Tissue Fibrosis Analysis

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Slices of penile tissue (5 µm) were fixed in 4% paraformaldehyde overnight to assess the level of fibrosis as determined using the Masson’s Trichrome Staining Kit (D026-1-3; Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the instructions provided.
Zhao C., Chen W., Gong F., Wang W., Fan C., Li Y., Lan T., Wang W, & Yuan M. (2024). Nitro-oleic acid (NO2-OA) ameliorates erectile dysfunction in a rat model of diabetes mellitus via modulation of fibrosis, inflammation and autophagy. Translational Andrology and Urology, 13(4), 537-547.
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3

Histological Evaluation of Lung Tissue

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Mice were euthanized, the lungs were harvested, fixed in 4% neutral buffered formalin, and paraffin sections were prepared. Paraffin-embedded sections were deparaffinized as previously described (Gong et al., 2019 (link)), then stained with hematoxylin and eosin (H&E, Beyotime) to evaluate changes in lung morphology or with a Masson's trichrome staining kit (Nanjing Jiancheng) to visualize collagen in lung tissue.
Liu P., Li Y., Li M., Zhou H., Zhang H., Zhang Y., Xu J., Xu Y., Zhang J., Xia B., Cheng H., Ke Y, & Zhang X. (2022). Endothelial Shp2 deficiency controls alternative activation of macrophage preventing radiation-induced lung injury through notch signaling. iScience, 25(3), 103867.
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4

Histological Analysis of Myocardial Tissue

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The paraffin-embedded myocardial tissues were cut into 5-μm thick sections and stained with H&E (hematoxylin and eosin staining kit, Beyotime, Shanghai, China) or Masson’s trichrome staining kit (Nanjing Jiancheng Technology Co., Ltd., Nanjing, China). The discrepant areas were captured by a DMI4000B fluorescence microscope (Leica, Munich, Germany).
Tang H., Zhong H., Liu W., Wang Y., Wang Y., Wang L., Tang S, & Zhu H. (2022). Melatonin Alleviates Hyperglycemia-Induced Cardiomyocyte Apoptosis via Regulation of Long Non-Coding RNA H19/miR-29c/MAPK Axis in Diabetic Cardiomyopathy. Pharmaceuticals, 15(7), 821.
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5

Evaluating Hepatic Steatosis and Fibrosis

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ORO staining was performed to evaluate hepatic steatosis. The ORO working solution was prepared by diluting the ORO stock solution (5 g/L in isopropanol) with distilled H2O at a ratio of 3 : 2. Liver sections were fixed in 60% isopropanol and stained with ORO working solution for 15 min. The stained lipid droplets were observed under a light microscope.
Fibrosis was assessed using Masson's trichrome staining kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), following the manufacturer's protocol. The sections were then visualized under a light microscope.
Tang L., Yu X., Zou L., Li S., Cheng Y., Wu Y., Xing L, & Fang H. (2023). Qinggan Huoxue Recipe Protects against Experimental Alcoholic Liver Fibrosis through CXCL16 Inhibition. Evidence-based Complementary and Alternative Medicine : eCAM, 2023, 5642713.
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6

Histological Analysis of Myocardial Tissues

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The para n-embedded myocardial tissues were cut into 5-μm thick sections and stained with H&E (hematoxylin and eosin staining kit, Beyotime, China) or Masson's trichrome staining kit (Nanjing Jiancheng Technology Co., Ltd., China). The discrepant areas were captured by a DMI4000B uorescence microscope (Leica, USA).
Tang H., Zhong H., Liu W., Hua T., Wang Y., Li Z., Chen Y., Dan Z., Wu D., Zhou Q., Wang Y., Wang L., Li H., Tang S., & Zhu H. (2021). Melatonin alleviates hyperglycemic-induced cardiomyocyte apoptosis via regulation of long non-coding RNA H19/miR-29c/MAPK axis in diabetic cardiomyopathy.
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7

Inhibition of Fibrosis Pathways

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HE, PAS and Masson's trichrome staining kits were purchased from Nanjing Jiancheng Biology Engineering Institute (Nanjing, Jiangsu, China). Dulbecco's modified Eagle's medium (DMEM), foetal bovine serum (FBS), penicillin and streptomycin were purchased from GIBCO BRL. Thrombin and dexamethasone powder were from Sigma‐Aldrich Co. Dabigatran was obtained from Boehringer Ingelheim Pharma. Inhibitors used were PAR1 antagonist SCH79797 (Tocris Bioscience), RhoA inhibitor Y‐16, ROCK inhibitor Y‐27632, YAP inhibitor verteporfin (MedChemExpress) and the actin polymerization inhibitor Cytochalasin D (Abcam). The antibodies used were anti‐collagen I, anti‐CTGF, anti‐β‐actin, anti‐GAPDH (Biosynthesis Biotechnology); anti‐RhoA, anti‐ROCK, anti‐phosphorylated MLC2, anti‐MLC2, anti‐phosphorylated SMAD2, anti‐SMAD2, anti‐phosphorylated YAP, anti‐α‐SMA, anti‐cyclin D1 (Cell Signaling Technology) and anti‐YAP (Santa Cruz Biotechnology).
Deng Z., Xie H., Cheng W., Zhang M., Liu J., Huo Y., Liao Y, & Cheng Y. (2020). Dabigatran ameliorates airway smooth muscle remodeling in asthma by modulating Yes‐associated protein. Journal of Cellular and Molecular Medicine, 24(14), 8179-8193.
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8

Baicalin Alleviates Bleomycin-Induced Lung Fibrosis

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Baicalin (purity > 85%) was supplied by Xi’an Watson Biotechnology Co., Ltd. (Xi’an, China). Bleomycin hydrochloride was obtained from Nippon Kayaku Co. (Tokyo, Japan) and was dissolved in saline (2.5 mg in 1 mL of saline). Prednisolone acetate was purchased from Zhejiang Xianju Pharmaceutical Co., Ltd. (Zhejiang, China). HYP, MDA, and SOD assay kits and Masson’s trichrome staining kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Methanol, acetonitrile and formic acid (HPLC grade) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Leucine enkephalin was purchased from Sigma-Aldrich (St. Louis, MO, USA). Deionised water was produced using a Milli-Q® Ultrapure Water System (Millipore, Billerica, USA).
Chang H., Meng H.Y., Bai W.F, & Meng Q.G. (2021). A metabolomic approach to elucidate the inhibitory effects of baicalin in pulmonary fibrosis. Pharmaceutical Biology, 59(1), 1014-1023.
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9

Histological Evaluation of Prostate Tissue

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Investigators were blinded to group allocation in animal tissue pathological examination. For histologic examination, the prostate tissue from each animal were fixed in neutral 10% formalin for 24 h, embedded in paraffin and cut into 4-µm-thick sections, then stained with H&E. Masson’s Trichrome staining kits (Catalog number: D026) were purchased from Nanjing Jiancheng Bioengineering Institute and performed according to manufacturer’s instructions. Immunohistochemistry and immunoflurocence were performed as previously described [54 (link)].
Cheng Y., Gao X.H., Li X.J., Cao Q.H., Zhao D.D., Zhou J.R., Wu H.X., Wang Y., You L.J., Yang H.B., He Y.L., Li Y.R., Bian J.S., Zhu Q.Y., Birnbaumer L, & Yang Y. (2018). Depression promotes prostate cancer invasion and metastasis via a sympathetic-cAMP-FAK signaling pathway. Oncogene, 37(22), 2953-2966.
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10

Histologic Fibrosis Scoring in Rats

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The qualitative histologic fibrosis score was determined according to the method Theiss et al. described in [22 (link)]. The scoring criteria are given in Table 1. The degree of collagen deposition was assessed by Masson's trichrome staining kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). About 2 sections for each rat were scored.
Zhao M., Weng Z., Huang Y., Zheng H., Han D., Shen J., Huang R., Liu H, & Wu L. (2021). Herb-Partitioned Moxibustion Improves Crohn's Disease-Associated Intestinal Fibrosis by Suppressing the RhoA/ROCK1/MLC Pathway. Evidence-based Complementary and Alternative Medicine : eCAM, 2021, 2247953.
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