Two baseline periods were obtained. Thereafter, either uroguanylin or fenoldopam (fenoldopam mesylate; 1659, Tocris Bioscience, Bristol, UK) was infused (1.0 μg/kg per minute) for 2 time periods, followed by 1 recovery period in which the drug infusion was stopped but the vehicle infusion was continued for another 40 minutes.
Fenoldopam
Manufactured by Bio-Techne
Sourced in United Kingdom, Germany
Fenoldopam is a dopamine D1 receptor agonist, primarily used as a vasodilator in the treatment of hypertension. It functions by activating dopamine receptors in the kidneys, resulting in vasodilation and increased renal blood flow, which can lead to a reduction in blood pressure.
Automatically generated - may contain errors
Lab products found in correlation
Sb431542, by Cayman Chemical (1 mentions)
Forskolin, by Cayman Chemical (1 mentions)
Formoterol, by Cayman Chemical (1 mentions)
A68930, by Bio-Techne (1 mentions)
Dihydrexidine, by Bio-Techne (1 mentions)
A 77636, by Bio-Techne (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Salbutamol, by Cayman Chemical (1 mentions)
Recombinant human tgf β1, by Thermo Fisher Scientific (1 mentions)
Y 27632, by Cayman Chemical (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Ropinirole, by Bio-Techne (1 mentions)
3 protocols using fenoldopam
1
Uroguanylin and Fenoldopam Infusion
2
Pharmacological Compounds for Cell Experiments
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Y-27632, forskolin, SB431542, formoterol, fenoterol, salbutamol, salmeterol, and fenoldopam were purchased from Cayman Chemical (Ann Arbor, MI). Dihydrexidine, A77636, A68930, and fenoldopam were purchased from Tocris Bioscience (Minneapolis, MN). Recombinant human TGF-β1 was purchased from PeproTech (Rocky Hill, NJ). Bovine serum albumin (BSA), 10% formalin and triton X-100 were purchased from Sigma-Aldrich (St. Louis, MO). Phosphate buffered saline (PBS) was purchased from Life Technologies (Carlsbad, CA).
3
Dopamine Agonists and Osteoblast Function
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To study the effect of dopamine on primary osteoblasts, cells were seeded in a 24-well plate (10 × 103 cells/well), cultured until they reached 80–90% confluence in complete culture medium and then treated with specific agonists of D1-like receptors (Fenoldopam, Tocris Bioscience, Wiesbaden-Nordenstadt, Germany) or D2-like receptors (Ropinirole, Tocris Bioscience, Wiesbaden-Nordenstadt, Germany) at different concentrations for 24 h. Afterwards, supernatants were collected and frozen in aliquots at −80 °C until analysis.
To study the effect of dopamine on activated osteoblasts, cells were cultured following the mineralization protocols described above. After 3 weeks, cells were treated with dopamine agonists in fresh medium for further 24 h. Afterwards, supernatants were collected and frozen in aliquots at −80 °C until analysis.
The concentrations chosen for Fenoldopam and Ropinirole were around the concentration described to have the best binding affinity, ±5–10× and had no toxic effects on the cells.
To study the effect of dopamine on activated osteoblasts, cells were cultured following the mineralization protocols described above. After 3 weeks, cells were treated with dopamine agonists in fresh medium for further 24 h. Afterwards, supernatants were collected and frozen in aliquots at −80 °C until analysis.
The concentrations chosen for Fenoldopam and Ropinirole were around the concentration described to have the best binding affinity, ±5–10× and had no toxic effects on the cells.
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