Akt pan 40d4 mouse mab

Manufactured by Cell Signaling Technology

AKT (pan; 40D4) mouse mAb is an antibody that specifically recognizes the AKT protein, also known as Protein Kinase B (PKB). AKT is a serine/threonine protein kinase that plays a crucial role in cellular processes such as cell proliferation, survival, and metabolism. This antibody can be used to detect and study the expression and activation of AKT in various biological samples.

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Lab products found in correlation

Peroxidase affinipure goat anti mouse igg, by Jackson ImmunoResearch (2 mentions) Peroxidase affinipure goat anti rabbit igg, by Jackson ImmunoResearch (2 mentions) Anti ha antibody, by Thermo Fisher Scientific (1 mentions) Irdye 800cw streptavidin antibody, by LI COR (1 mentions) Alexa fluor 568 goat anti rabbit igg, by Abcam (1 mentions) Phospho akt ser473 d9e xp rabbit mab, by Cell Signaling Technology (1 mentions) Phospho p44 42 mapk erk1 2 thr202 tyr204 antibody, by Cell Signaling Technology (1 mentions) Phospho akt ser473 193h12 rabbit mab, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Pan-AKT (169 citations) Akt-pan (40D4) (7 citations) AKT (40D4, (2 citations)

3 protocols using akt pan 40d4 mouse mab

AGR2 Interactome Identification and Analysis

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Biotinylation, AGR2 IP and AGR2 BioID elution were verified by Western blot analysis with IRDye^® 800 CW Streptavidin antibody (Licor) 1:1000 dilution in Odysee^® blocking buffer (Licor). Normal mouse IgG CNTRL antibody for IP (Cat# sc-2342), AGR2 antibody (6C5) (Cat# sc-101211), were purchased from Santa Cruz. Phospho-YAP (Ser127) (D9W21) (Cat#13008), YAP (D8H1X) XP^® Rabbit mAb (Cat#14074), Phospho-RPS6 rabbit (Ser240/244) (Cat#2215), RPS6 (5G10) rabbit mAB (Cat#2217), RPS6 (54D2) mouse mAb (Cat#2317), p70 S6 kinase (49D7) rabbit mAB (Cat#2708), phosphor-p70 S6 kinase (Thr389) (108D2) rabbit mAB (Cat#9234), Rictor (53A2) Rabbit mAb (Cat#2114), phospho-Rictor (Thr1135) (D30A3) rabbit mAb (Cat#3806), Akt (pan) (40D4) mouse mAb (Cat#2920), phosphor-Akt (Ser473) (D9E) rabbit mAb (Cat#4060) were purchased from Cell Signaling Technologies. Alpha-Tubulin (DM1A) mouse mAb (Cat#T9026) and beta-Actin (AC-15) mouse mAb (Cat#A1978) were purchased from Sigma-Aldrich. AntiHA antibody was purchased from Thermo (26183-BTIN). A second AGR2 antibody (Cat#2533–1) was obtained from Epitomics and used for ELISA and western blot.

Tiemann K., Garri C., Lee S.B., Malihi P.D., Park M., Alvarez R.M., Yap L.P., Mallick P., Katz J.E., Gross M.E, & Kani K. (2018). Loss of ER retention motif of AGR2 can impact mTORC signaling and promote cancer metastasis. Oncogene, 38(16), 3003-3018.

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Immunoblotting Antibody Reagents for Signal Transduction

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Phospho AKT (Ser473, D9E) XP rabbit mAb, phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) antibody, HER2/ErbB2 antibody, and AKT (pan; 40D4) mouse mAb were purchased from Cell Signaling Technology (Danvers, MA). ERK 1 (C-19) antibody was from Santa Cruz Biotechnology, Inc. (Dallas, TX), and Anti-Pan-Ras (Ab-3) mouse mAb was from Sigma-Aldrich (St. Louis, MO). Anti-laminin 5 antibody and Alexa Fluor 568 goat anti-rabbit IgG were from Abcam (Cambridge, UK). Rictor mouse mAb 1G3P2C9 was obtained from Bethyl Laboratories (Montgomery, TX). Peroxidase AffiniPure goat anti-mouse IgG, peroxidase AffiniPure goat anti-rabbit IgG, and AMCA AffiniPure goat anti-mouse IgG were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA).

Collins S.E., Wiegand M.E., Werner A.N., Brown I.N., Mundo M.I., Swango D.J., Mouneimne G, & Charest P.G. (2023). Ras-mediated activation of mTORC2 promotes breast epithelial cell migration and invasion. Molecular Biology of the Cell, 34(2), ar9.

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Antibodies for AKT, MAPK, and Ras Signaling

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Phospho-AKT (Ser473; 193H12) rabbit mAb, AKT (pan; 40D4) mouse mAb, and P-p44/42 MAPK (T202/Y204; pERK) rabbit mAb were purchased from Cell Signaling Technology (Danvers, MA). ERK1 (C-16) IgG rabbit mAb was purchased from Sant Cruz Biotechnology (Dallas, TX), and anti-Pan-Ras (Ab-3) Mouse Ab (Ras 10) was from MilliporeSigma (Burlington, MA). Rabbit anti-Rictor Affinity Purified mAb was purchased from Bethyl Laboratories (Montgomery, TX). Peroxidase AffiniPure Goat anti-Rabbit IgG and peroxidase AffiniPure Goat anti-Mouse IgG were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA).

Werner A.N., Kumar A.I, & Charest P.G. (2023). CRISPR-mediated reversion of oncogenic KRAS mutation results in increased proliferation and reveals independent roles of Ras and mTORC2 in the migration of A549 lung cancer cells. Molecular Biology of the Cell, 34(13), ar128.

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