The instruments used include a horizontal decolorization shaker (Beijing Dingguo Biotechnology Co, Ltd.), an ultrapure water machine (Shanghai Hetai Instruments Co., Ltd.), a low-temperature high-speed centrifuge (Eppendof, Germany), a pipette (Biosharp, China), a −80°C cryogenic refrigerator (Japan), a transmission electron microscope (JEM-1400, Japan), a special imaging system for transmission electron microscope (Gatan, USA), a microplate reader (BioRad, USA), an electrophoresis apparatus (BioRad, USA), an electrophoresis tank (BioRad, USA), and a gel imaging system (BioRad, USA). The BCA Protein Quantification Kit, Animal Tissue Mitochondria Isolation Kit, Rat Citrate Synthase (CS) Enzyme-linked Immunosorbent Assay (ELISA) Kit, and Respiratory Chain Complex II ELISA Kit were purchased from Beyotime Institute of Biotechnology, Shanghai, China. Anti-GAPDH and anti-Cox I were from Abcam (Nanjing).
Animal tissue mitochondria isolation kit
Manufactured by Beyotime
Sourced in China
The Animal Tissue Mitochondria Isolation Kit is a laboratory product designed to isolate mitochondria from animal tissue samples. It provides a standardized protocol and reagents to effectively extract and purify mitochondria for further analysis and experimentation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using animal tissue mitochondria isolation kit
1
Mitochondrial Function Assessment Protocol
2
Ultrastructural Analysis of Rat Skeletal Muscle
Check if the same lab product or an alternative is used in the 5 most similar protocols
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1. Electron microscopy sample preparation: The rats were weighed in batches and anesthetized by intraperitoneal injection with 3% pentobarbital (30 mg/kg). A blade was used to rapidly separate a small piece of rat pear Longus longitudinally along the muscle fiber into 2×1×1 mm3 and then pre-chilled in 2.5% glutaraldehyde at 4°C for electron microscopy.
2. Preparation of skeletal muscle mitochondria: Each muscle tissue sample (100 mg) was placed in a pre-chilled lysis solution and analyzed using the Beyotime Animal Tissue Mitochondria Isolation Kit.
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