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β-tubulin (E7) is a monoclonal antibody produced by the Developmental Studies Hybridoma Bank. It is a specific marker for the beta-tubulin protein, which is a key component of microtubules in eukaryotic cells.

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Lab products found in correlation

Gapdh gapdh 71.1, by Merck Group (1 mentions) Tnfr1, by Cell Signaling Technology (1 mentions) On target smart pool, by Thermo Fisher Scientific (1 mentions) Anti tnfα xt3, by BioXCell (1 mentions) Casp8, by Thermo Fisher Scientific (1 mentions) Irdye 680lt donkey anti mouse igg, by LI COR (1 mentions) Irdye 800cw donkey anti rabbit igg, by LI COR (1 mentions) Anti ddk flag, by OriGene (1 mentions) Phospho stat3 y705, by Cell Signaling Technology (1 mentions) Odyssey classic, by LI COR (1 mentions) Stat3, by Cell Signaling Technology (1 mentions)

2 protocols using β tubulin e7

1

Enhancing Oncolytic Virus Therapy

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Cells were transfected with combinations of non-targeting (NT), Receptor Interacting Protein1 (RIP1), Caspase-8 (CASP8), TNF-R1, and DR5 small interfering RNA (siRNA) (ON-Target SmartPool, Thermo Fisher Scientific) for 48 hr. Cells were then treated with vehicle or 5 μM LCL161 and infected with the indicated OV. Cell viability was assessed by Alamar blue. Knockdown of TNF-R1 and DR5 was determined by western blotting using the following antibodies: RIP1 (D94C12; Cell Signaling Technology), CASP8 (AF705), DR5 (3696; Cell Signaling Technology), TNF-R1 (3736; Cell Signaling Technology), β-tubulin (E7; Developmental Studies Hybridoma Bank), and GAPDH (GAPDH-71.1; Sigma). For antibody-mediated neutralization of TNF-α, cells were treated with 50 μg/mL of mouse immunoglobulin G (IgG) control (HRPN; BioXCell) or anti-TNF-α (XT3.11; BioXCell). One hour later, cells were treated with vehicle or 5 μM LCL161 and the indicated MOI of OV. Cell viability was assessed by Alamar blue.
Beug S.T., Pichette S.J., St-Jean M., Holbrook J., Walker D.E., LaCasse E.C, & Korneluk R.G. (2018). Combination of IAP Antagonists and TNF-α-Armed Oncolytic Viruses Induce Tumor Vascular Shutdown and Tumor Regression. Molecular Therapy Oncolytics, 10, 28-39.
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2

Western Blot Protein Analysis

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Primary antibodies used were: Anti-DDK (FLAG) (Origene, TA50011), Phospho-Y705-STAT3, STAT3, GFP (Cell Signaling Technology, #9145, #9139, and #2956), and β-tubulin E7 (Developmental Studies Hybridoma Bank) and infrared secondary antibodies from LI-COR (IRDye 800CW Donkey anti-Rabbit IgG and IRDye 680LT Donkey anti-Mouse IgG). Blots were developed and quantified on a LI-COR Odyssey Classic.
Jaslow S.L., Gibbs K.D., Fricke W.F., Wang L., Pittman K.J., Mammel M.K., Thaden J.T., Fowler VG J.r., Hammer G.E., Elfenbein J.R, & Ko D.C. (2018). Salmonella activation of STAT3 signaling by SarA effector promotes intracellular replication and production of IL-10. Cell reports, 23(12), 3525-3536.
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