Optical rotations were measured with a Jasco P1000 digital polarimeter. UV spectra were recorded by a Hewlett Packard 8453 UV–vis spectrometer. CD spectra were obtained with a Jasco J-715 circular dichroism spectrophotometer. 1D and 2D NMR were performed on a VNS 600 MHz spectrometer operating at 600 MHz for protons and 150 MHz for carbon. Chemical shifts are expressed in ppm and referenced relative to the residual solvent signals. Mass spectra were recorded with a Micromass LCT mass spectrometer, and the lock mass calibration was applied to accurately measure masses. Semi-preparative HPLC was performed with an Agilent system consisting of a vacuum degasser, quaternary pump, diode array detector (DAD), and Luna 5u C18 (2) 100 Å column (250 × 10.00 mm; Phenomenex). Column chromatography (CC) was carried out on either silica gel (Merck KGaA, 70–230 mesh) or SephadexTM LH-20 (GE Healthcare Sweden). TLC was performed with precoated silica gel 60 F254 plates (Merck KGaA) and spots were visualized under UV light (254 and 365 nm) or by spraying with 20% H2SO4 and heating.
P1000 digital polarimeter
Manufactured by Jasco
The P1000 digital polarimeter is a laboratory instrument used to measure the optical rotation of a substance. It determines the angle at which plane-polarized light is rotated when passing through an optically active material. The P1000 provides precise and reliable measurements of optical activity, which is a fundamental property of many organic and inorganic compounds.
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2 protocols using p1000 digital polarimeter
1
Analytical Characterization of Natural Products
2
Polymer Characterization by NMR, SEC, and DSC
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1H NMR (400 MHz) measurements
were acquired at 27 °C on a Bruker Analytik DPX400 spectrometer
with tetramethylsilane (TMS) as an internal standard (0 ppm). The
number-average molecular weight (Mn) and
molecular weight distribution (Mw/Mn) of the polymers were measured by using a
size exclusion chromatography (SEC) system consisting of a JASCO PU-4185
pump system, a JASCO RI-4035 differential refractometer, a TSK-gel
supermultipore-M column, and a JASCO co-2065 plus intelligent column
oven (eluent, tetrahydrofuran; flow rate, 0.35 mL min–1; temperature, 40 °C; Tosoh Corp.), calibrated by a poly(styrene)
standard. Differential scanning calorimetry (DSC) measurements were
performed with a DSC7020 instrument (HITACHI). The heating was conducted
from 30 to 80 °C; the temperature was then cooled to −100
°C. The second scan was conducted from −90 to 80 °C.
The rate of temperature change was 10 °C min–1, and all measurements were performed under N2 gas flow.
The specific rotation ([α]D25) was measured in a THF solution (0.01 g mL–1) at 25 °C using a JASCO P-1000 digital polarimeter.
were acquired at 27 °C on a Bruker Analytik DPX400 spectrometer
with tetramethylsilane (TMS) as an internal standard (0 ppm). The
number-average molecular weight (Mn) and
molecular weight distribution (Mw/Mn) of the polymers were measured by using a
size exclusion chromatography (SEC) system consisting of a JASCO PU-4185
pump system, a JASCO RI-4035 differential refractometer, a TSK-gel
supermultipore-M column, and a JASCO co-2065 plus intelligent column
oven (eluent, tetrahydrofuran; flow rate, 0.35 mL min–1; temperature, 40 °C; Tosoh Corp.), calibrated by a poly(styrene)
standard. Differential scanning calorimetry (DSC) measurements were
performed with a DSC7020 instrument (HITACHI). The heating was conducted
from 30 to 80 °C; the temperature was then cooled to −100
°C. The second scan was conducted from −90 to 80 °C.
The rate of temperature change was 10 °C min–1, and all measurements were performed under N2 gas flow.
The specific rotation ([α]D25) was measured in a THF solution (0.01 g mL–1) at 25 °C using a JASCO P-1000 digital polarimeter.
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