17β-estradiol (E2) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium dodecyl sulfatepolyacrylamide gel electrophoresis and bicinchoninic acid assay (BCA) protein assay kit were provided by Beyotime Biotechnology (Shanghai, China). Rabbit anti-chemokine living 8 (CXCL8) was purchased from Biorbyt (Cambridge, UK). Rabbit anti-alpha-2a adrenergic receptor antibody (ADRA2A) was provided by Merck (Kenilworth, NJ, USA). Rabbit anti-islet amyloid polypeptide antibody, rabbit anti-melatonin receptor type 1B (MTNR1B) antibody, rabbit anti-FC-C chemokine receptor type 2 (CCR2) antibody, and mouse monoclonal beta-actin antibody were purchased from Abcam (Cambridge, UK). Enzyme-linked immunosorbent assay (ELISA) kits of bone-specific alkaline phosphatase (BALP) and β-C-terminal telopeptide of type I collagen (β-CTX) were provided by Shanghai Enzyme-linked Biotechnology (Shanghai, China). Rabbit antirunt-related transcription factor 2 (Runx2) polyclonal antibody was supplied by Boster Biological Technology (Wuhan, China). Cy3-AffiniPure goat anti-mouse IgG (H+L) and Cy3-AffiniPure goat anti-rabbit IgG (H+L) were purchased from Shanghai Yi Sheng Biotechnology (Shanghai, China).
Mouse monoclonal beta actin antibody
Manufactured by Abcam
Sourced in United Kingdom, United States
Mouse monoclonal beta-actin antibody is a primary antibody that specifically binds to beta-actin, a highly conserved cytoskeletal protein found in all eukaryotic cells. This antibody can be used to detect and quantify beta-actin expression in various applications such as Western blotting, immunocytochemistry, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
17β estradiol e2, by Merck Group (1 mentions)
Sodium dodecyl sulfate polyacrylamide gel electrophoresis, by Beyotime (1 mentions)
Bicinchoninic acid assay bca protein assay kit, by Beyotime (1 mentions)
Odyssey system, by LI COR (1 mentions)
Lamin b antibody, by Santa Cruz Biotechnology (1 mentions)
3 protocols using mouse monoclonal beta actin antibody
1
Molecular Profiling of Bone Metabolism
2
Western Blot Analysis of HA-Tagged Proteins
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Cell lysates were prepared with cOmplete Lysis M buffer, EDTA-free according to the manufacturer’s protocol (Roche). Protein samples were separated on 4–20% Mini-PROTEAN gels (Bio-Rad) and transferred to a polyvinylidene difluoride (PVDF) membrane. The blot was probed with rabbit monoclonal HA-Tag (C29F4) antibody (Cell Signaling Technology, #3724) and mouse monoclonal beta-actin antibody (Abcam, ab8226) and then was imaged by the LiCor Odyssey System.
3
Inflammatory Signaling Pathway Analysis
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Pheophytin-b was purchased from Wako Pure Chemical Industries, Ltd., Osaka, Japan. LPS (Escherichia coli O26:B6, L2654) was purchased from Sigma (St. Louis, MO, USA).
The NOS2 antibody was purchased from BD Biosciences (San Jose, CA, USA). Antibodies against p44/p42 MAP Kinase (Thr202/Tyr204), p38 MAP Kinase (Thr180/Tyr182), SAPK/JNK (Thr183/Tyr185), ERK1/2 MAP Kinase (Thr202/Tyr204), phospho-IκB-α (Ser32/36), STAT1 (Tyr701), Akt (Ser473), PI3K p85 (Tyr458)/p55 (Tyr199), c-Fos (9F6), c-Jun (Ser73), and COX-2 were all purchased from Cell Signaling (Danvers, MA, USA). The NF-κB p65 rabbit monoclonal antibody (Cell Signaling), lamin B antibody (Santa Cruz, CA, USA), and mouse monoclonal beta-Actin antibody (Abcam, Cambridge, MA, USA) were also utilized.
The NOS2 antibody was purchased from BD Biosciences (San Jose, CA, USA). Antibodies against p44/p42 MAP Kinase (Thr202/Tyr204), p38 MAP Kinase (Thr180/Tyr182), SAPK/JNK (Thr183/Tyr185), ERK1/2 MAP Kinase (Thr202/Tyr204), phospho-IκB-α (Ser32/36), STAT1 (Tyr701), Akt (Ser473), PI3K p85 (Tyr458)/p55 (Tyr199), c-Fos (9F6), c-Jun (Ser73), and COX-2 were all purchased from Cell Signaling (Danvers, MA, USA). The NF-κB p65 rabbit monoclonal antibody (Cell Signaling), lamin B antibody (Santa Cruz, CA, USA), and mouse monoclonal beta-Actin antibody (Abcam, Cambridge, MA, USA) were also utilized.
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