Erythromycin enteric-coated tablets (H42021990, Yichang Humanwell Pharmaceutical Co., LTD.); Vorinostat Capsules (180509, Beijing Hengrui Kangda Medical Science and Technology Development Co., Ltd.); Budesonide (AstraZeneca 8339000); Rabbit Anti-Collagen III Polyclonal Antibody (bs-10423R, Bioss); Rabbit Anti-Collagen I Polyclonal Antibody (bs-0549R, Bioss); Rabbit Anti-HDAC2 Polyclonal Antibody (bs-1813R, Bioss); Rabbit VEGF ELISA kit (MM-021001); Rabbit TGF-β1 ELISA kit (MM-3684001); Rabbit Polyclonal Anti-VEGF (bs-1313R, Bioss, 1/500–1/2000); Rabbit Polyclonal Anti-TGFβ1 (bs-0086R, Bioss, 1/500–1/2000); Rabbit monoclonal Anti-IL-8 (ab34100, abcam, 1/1000); Rabbit Polyclonal Anti-HDAC2 (OmnimAbs, OM105905, 1/500–1/2000); fluorescence microscope (CKX53, OLYMPUS); Microplate Reader (RT-6100, Rayto); Protein vertical electrophoresis instrument (DYY-6C, Beijing 61 instrument factory); Ultra High Sensitivity Chemiluminescence Imaging System (Chemi DocTM XRS+, Bio-Rad Shanhhai Laboratories).
Ab34100
Manufactured by Abcam
Ab34100 is a laboratory equipment product. It is a precision instrument designed for laboratory use. The core function of this product is to perform specific tasks required in a research or analytical setting. No further details about its intended use or capabilities are provided.
Automatically generated - may contain errors
Lab products found in correlation
Bs 0086r, by Bioss Antibodies (1 mentions)
Bs 0549r, by Bioss Antibodies (1 mentions)
Bs1813r, by Bioss Antibodies (1 mentions)
Bs 10423r, by Bioss Antibodies (1 mentions)
Bs 1313r, by Bioss Antibodies (1 mentions)
Ckx53, by Olympus (1 mentions)
Rt 6100, by Rayto (1 mentions)
Budesonide, by AstraZeneca (1 mentions)
Ab199013, by Abcam (1 mentions)
Ab8226, by Abcam (1 mentions)
2 protocols using ab34100
1
Collagen and HDAC2 Regulation in Angiogenesis
2
Quantitative Assessment of Inflammatory Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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IL-8, TNF-α, CXCL5 and β-actin mRNA levels were determined by quantitative reverse transcription PCR (qRT-PCR)[21 (link)]. The primers used were as follows: IL-8 forward primer (5’- ATG ACT TCC AAG CTG GCC GTG GCT-3’ ) and reverse primer (5’-TCT CAG CCC TCT TCA AAA ACT TCT C -3’ ); TNF-α forward primer (5’- CGA GTG ACA AGC CTG TAG C -3’ ) and reverse primer (5’-CCT TCT CCA GCT GGA GAG C-3’ ); CXCL5 forward primer (5’-GAG AGC TGC GTT GCG TTT G-3’ ) and reverse primer (5’-TTT CCT TGT TTC CAC CGT CCA-3’ ); GAPDH forward primer (5’-ACCACAGTCCATGCCATCAC-3’ ) and reverse primer (5’-TCCACCACCCTGTTGCTGTA-3’ ). Sample input was normalized to β-actin mRNA expression. IL-8, TNF-α, CXCL5 and β-actin protein levels were determined by western blot analysis[22 (link)], using following antibodies: ab34100 (1:1000 dilution, abcam, Cambridge, MA) for IL-8, ab199013 (1:1000 dilution, abcam, Cambridge, MA) for TNF-α, ENA-78 (1:1000 dilution, Santa Cruz Biotechnology, Santa Cruz, CA) for CXCL5 and ab8226 (1:500 dilution, abcam, Cambridge, MA) for β-actin.
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