Horseradish peroxidase pod

Manufactured by Roche

Sourced in Germany

Horseradish peroxidase (POD) is an enzyme commonly used in various laboratory applications. It catalyzes the oxidation of substrates in the presence of hydrogen peroxide. POD is a versatile tool employed in a range of assays and detection methods.

Automatically generated - may contain errors

Lab products found in correlation

Superfrost plus slide, by Thermo Fisher Scientific (2 mentions) Anti cleaved caspase 9, by Cell Signaling Technology (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Rabbit anti b cell lymphoma 2 bcl 2, by Cell Signaling Technology (1 mentions) Enhanced chemiluminescent detection system, by GE Healthcare (1 mentions) In situ cell death detection kit, by Roche (1 mentions) Goat anti mouse igg, by Abcam (1 mentions) Ab8055, by Abcam (1 mentions) Ti e fluorescence microscope, by Nikon (1 mentions) Cm3050 cryostat, by Thermo Fisher Scientific (1 mentions) Nis elements software, by Nikon (1 mentions)

2 protocols using horseradish peroxidase pod

Evaluating Apoptosis-Related Protein Markers

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Human recombinant Hsp72 (low endotoxin) was obtained from Enzo Life Science (Farmingdale, NY, USA). Rabbit anti-B-cell lymphoma 2 (Bcl-2), anticleaved caspase-3, and anticleaved caspase-9 antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Goat anti-rabbit immunoglobulin G [28 (link)] was obtained from Jackson ImmunoResearch Laboratories (West Grove, PA, USA), goat anti-mouse IgG was purchased from Abcam (Cambridge, MA, USA), and rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) monoclonal and mouse anti-Bcl-2-associated X protein (Bax) polyclonal antibodies were obtained from Santa Cruz Biotechnology, Inc. (Delaware Avenue, California, USA). Rabbit anticleaved poly (ADP-ribose) polymerase (PARP-1) polyclonal antibody was obtained from Bioworld Technology, Inc. (Minneapolis, MN, USA). Enhanced chemiluminescent detection system was obtained from Pharmacia Biotech (Välinge, Sweden). In situ cell death detection kit and horseradish peroxidase (POD) were purchased from Roche Applied Science (Mannheim, Germany). Other chemicals and reagents were of analytical grade.

Tsai T.N., Ho J.J., Liu M.S., Lee T.Y., Lu M.C., Liu C.J., Huang L.J., Lue S.I, & Yang R.C. (2015). Role of Exogenous Hsp72 on Liver Dysfunction during Sepsis. BioMed Research International, 2015, 508101.

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Multi-omic Colon and Ileum Analysis

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For detailed protocol, see Supplementary methods. Briefly, 12 μm sections of mouse colon and ileum were cut using a Leica CM3050 cryostat and mounted on Superfrost Plus slides (Thermo Fisher Scientific) and allowed to air-dry for an hour. Sections were hybridised with DIG-labelled Insl5 or Rxfp4 probe (1:100 each) overnight at 60°C, washed and incubated with horseradish peroxidase-(POD, 1:500, Roche) conjugated anti-DIG antibody before tyramide signal amplification with FITC-tyramide. Following in situ hybridisation, sections were blocked (2% BSA, 0.1% (v/v) Tween-20 in PBS), incubated with rabbit anti-proglucagon antibody (1:100, ab8055, Abcam) overnight, washed with TBST and incubated with anti-rabbit Alexa594 secondary antibody (1:500, Thermo Fisher Scientific). Fluorescent wide-field images were acquired by tile-scanning using a Nikon Ti-E fluorescence microscope with a 20× objective and photo-stitched during acquisition in NIS-Elements software (Nikon Instruments).

Ang S.Y., Evans B.A., Poole D.P., Bron R., DiCello J.J., Bathgate R.A.D., Kocan M., Hutchinson D.S, & Summers R.J. (2018). INSL5 activates multiple signalling pathways and regulates GLP-1 secretion in NCI-H716 cells. Journal of molecular endocrinology, 60(3).

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