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2 protocols using acetylcholine chloride

1

Acetylcholine Chloride Tissue Stimulation

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For chemical stimulation, acetylcholine chloride (011-00592, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was used. It was dissolved in Dulbecco’s PBS (048-29805, FUJIFILM Wako Pure Chemical Corporation) to the desired concentration which avoids expansion of tissue due to osmotic pressure. PBS is widely used in experiments treating tissue.
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2

Synthesis of Amine-Functionalized Benzofuran Compounds

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Benzofuran-2-boronic acid, diEthylamine, pyrrole, ethanolamine and benzylethanolamine were purchased from Tokyo Chemical Industries (Tokyo, Japan). Palladium(II) acetate (Pd(OAc)2), palladium(II) nitrate (Pd(NO3)2), hexylamine and choline chloride were purchased from Nacalai Tesque (Kyoto, Japan). Ethylamine, aniline, diphenylamine, trimEthylamine, TEA, triphenylamine, acetylcholine chloride, L-carnitine, pyridine, piperidine, 1,3-diaminopropane, spermidine, spermine, tris{tris-[3,5bis (trifluoromethyl)phenyl]phosphine}palladium (Superstable Pd(0)), tetrakis(triphenylphosphine)palladium(0) (Pd(PPh3)4), and bis(dibenzylideneacetone)palladium(0) (Pd(dba)2) were purchased from Wako (Osaka, Japan). Acetonitrile (HPLC grade) and palladium(II) chloride (PdCl2) were from Kanto Chemical (Tokyo, Japan). Water was distilled and passed through a Pure Line WL21P system (Yamamoto, Tokyo, Japan). All other chemicals were of the highest purity and quality available. Stock solutions of amine were prepared in acetonitrile at the concentration of 500 μM each.
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