Fast panotic kit

Manufactured by Laborclin

Sourced in Brazil

The Fast Panotic Kit is a laboratory product manufactured by Laborclin. The core function of this kit is to perform a rapid staining procedure for the visualization of cells and microorganisms in biological samples.

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Lab products found in correlation

Methanol acetone, by Merck Group (1 mentions)

2 protocols using fast panotic kit

Giemsa Staining for Macrophage Infection

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After 4 and 24 h of infection, the chamber slides were washed two times with PBS 1× and were then fixed with acetone/methanol (1:1 v/v; Sigma-Aldrich, St. Louis, MO, USA) and were incubated at −20 °C for 24 h followed by Giemsa staining (Fast Panotic Kit, Laborclin, São Paulo, Brazil). At least 600 macrophages were counted per well to achieve the parameters of the Macrophage Infection Rate [(infected macrophage/total macrophage) ∗ 100], amastigotes per infected macrophage, and the infection index, which was calculated by multiplying the rate of infected macrophages by the average number of amastigotes per macrophage.

Acuña S.M., Zanatta J.M., de Almeida Bento C., Floeter-Winter L.M, & Muxel S.M. (2022). miR-294 and miR-410 Negatively Regulate Tnfa, Arginine Transporter Cat1/2, and Nos2 mRNAs in Murine Macrophages Infected with Leishmania amazonensis. Non-Coding RNA, 8(1), 17.

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Leukocyte Count in Temporomandibular Joint

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To analyze the total leukocyte count, 10 μl of EDTA (1 mM) were used to wash the articular cavity of the temporomandibular joint. Afterwards, 90 μl of PBS was added and the exudate was dilute in Türkś stain (1:2) and leukocytes were analyzed in a Neubauer chamber in an optical microscope (100 x). The results are expressed as cells x 10 4 per joint. For differential count (neutrophils and lymphocytes), joint exudate was submitted to cytocentrifugation and stained by fast Panotic kit (LaborClin, Pinhais, PR, Brazil) and were analyzed in an optical microscope (1000 x). The results are expressed as percentage of each cell type/total leukocyte counts x 10 4 per cavity.

Tarkany Basting R., Henrique Napimoga M., Antônio Trindade Silva C., Ballassini Abdalla H., Campos Durso B., Henrique Barboza Martins L., de Abreu Cavalcanti H., Hammock B.D, & Trindade Clemente-Napimoga J. (2023). Soluble epoxide hydrolase inhibitor blockage microglial cell activation in subnucleus caudalis in a persistent model of arthritis. International immunopharmacology, 120.

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