Fitc anti mouse ki67

Manufactured by BioLegend

FITC Anti-Mouse Ki67 is a reagent that can be used to detect the expression of the Ki67 protein in mouse cells. Ki67 is a nuclear protein that is associated with cellular proliferation. This antibody is conjugated to the fluorescent dye FITC, which allows for the detection of Ki67-positive cells using flow cytometry or fluorescence microscopy.

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Lab products found in correlation

Facscalibur flow cytometer, by BD (3 mentions) Anti mouse cd25 apc, by Thermo Fisher Scientific (3 mentions) Anti mouse cd4 pe, by BD (3 mentions) Fixation permeabilization solution, by BD (3 mentions) Percp anti mouse cd44, by BioLegend (3 mentions) Pe anti mouse cd62l, by BD (3 mentions) Anti mouse cd4 fitc, by Thermo Fisher Scientific (3 mentions) Lymphocyte isolate medium, by Keygen Biotech (3 mentions) Ow cytometry antibodies, by BD (2 mentions) Flow cytometry antibodies, by BD (1 mentions)

Spelling variants of this product

Anti-Ki67 (27 citations) Anti-Ki67-FITC (9 citations) Ki67-FITC (8 citations) Anti-mouse Ki67 (2 citations)

3 protocols using fitc anti mouse ki67

Flow Cytometry Analysis of T Cell Subsets

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For flow cytometry, fresh blood was collected in anticoagulant tubes (BD, 8215736) and peripheral blood mononuclear cells (PBMC) were obtained by lymphocyte isolate medium (KeyGEN BioTECH, kga831). Cells were washed and treated with the Fixation/Permeabilization Solution (BD) for Ki67 detection, incubated with flow cytometry antibodies (BD Pharmingen), and resuspended in phosphate-buffered saline (PBS) with 1% bovine serum albumin (BSA). Then cells were determined with BD FACS Calibur Flow Cytometer. Antibody collocations were as follows:

(i)

CD4⁺ CD25⁺ T cells: FITC Anti-Mouse CD4 (eBioscience, clone: RM4–4), APC Anti-Mouse CD25 (eBioscience, clone: PC61.5);

(ii)

CD4⁺ CD44⁺ T cells: FITC Anti-Mouse CD4 (eBioscience, clone: RM4–4), PerCP Anti-Mouse CD44 (BioLegend, clone: IM7);

(iii)

CD4⁺ CD62L⁺ T cells: FITC Anti-Mouse CD4 (eBioscience, clone: RM4–4); PE Anti-Mouse CD62L (BD, clone: MEL-14);

(iv)

CD4⁺ Ki67⁺ T cells: PE Anti-Mouse CD4 (BD, clone: RM4–5); FITC Anti-Mouse Ki67 (BioLegend, clone: 16A8).

Zhou L., Long J., Sun Y., Chen W., Qiu R, & Yuan D. (2020). Resveratrol ameliorates atherosclerosis induced by high-fat diet and LPS in ApoE−/− mice and inhibits the activation of CD4+ T cells. Nutrition & Metabolism, 17, 41.

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Flow Cytometric Immunophenotyping of T Cell Subsets

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For ow cytometry, fresh blood was collected in anticoagulant tubes (BD, 8215736) and peripheral blood mononuclear cells (PBMC) were obtained by lymphocyte isolate medium (KeyGEN BioTECH, kga831).
Cells were washed and treated with the Fixation/Permeabilization Solution (BD) for Ki67 detection, incubated with ow cytometry antibodies (BD Pharmingen), and resuspended in phosphate-buffered saline (PBS) with 1% bovine serum albumin (BSA). Then cells were determined with BD FACS Calibur Flow Cytometer. Antibody collocations were as follows:
i. CD4 + CD25 + T cells: FITC Anti-Mouse CD4 (eBioscience, clone: RM4-4), APC Anti-Mouse CD25 (eBioscience, clone: PC61.5);
ii. CD4 + CD44 + T cells: FITC Anti-Mouse CD4 (eBioscience, clone: RM4-4), PerCP Anti-Mouse CD44
(BioLegend, clone: IM7);
iii. CD4 + CD62L + T cells: FITC Anti-Mouse CD4 (eBioscience, clone: RM4-4); PE Anti-Mouse CD62L (BD, clone: MEL-14); iv. CD4 + Ki67 + T cells: PE Anti-Mouse CD4 (BD, clone: RM4-5); FITC Anti-Mouse Ki67 (BioLegend, clone:
16A8).

Zhou L., Long J., Sun Y., Chen W., Qiu R., & Yuan D. (2020). Resveratrol Ameliorates Atherosclerosis Induced by High-fat Diet and LPS in ApoE-/- Mice and Inhibits the Activation of CD4+ T Cells by Regulating the Expression of Dnmt.

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Multiparametric Flow Cytometry of T Cells

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For ow cytometry, fresh blood was collected in anticoagulant tubes (BD, 8215736) and peripheral blood mononuclear cells (PBMC) were obtained by lymphocyte isolate medium (KeyGEN BioTECH, kga831). Cells were washed and treated with the Fixation/Permeabilization Solution (BD) for Ki67 detection, incubated with ow cytometry antibodies (BD Pharmingen), and resuspended in phosphate-buffered saline (PBS) with 1% bovine serum albumin (BSA). Then cells were determined with BD FACS Calibur Flow Cytometer. Antibody collocations were as follows: CD4 + CD25 + T cells: FITC Anti-Mouse CD4 (eBioscience, clone: RM4-4), APC Anti-Mouse CD25 (eBioscience, clone: PC61.5); CD4 + CD44 + T cells: FITC Anti-Mouse CD4 (eBioscience, clone: RM4-4), PerCP Anti-Mouse CD44 (BioLegend, clone: IM7); CD4 + CD62L + T cells: FITC Anti-Mouse CD4 (eBioscience, clone: RM4-4); PE Anti-Mouse CD62L (BD, clone: MEL-14); CD4 + Ki67 + T cells: PE Anti-Mouse CD4 (BD, clone: RM4-5); FITC Anti-Mouse Ki67 (BioLegend, clone: 16A8).

Zhou L., Long J., Sun Y., Chen W., Qiu R., & Yuan D. (2020). Resveratrol Ameliorates Atherosclerosis Induced by High-fat Diet and LPS in ApoE-/- Mice and Inhibits the Activation of CD4+ T Cells.

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