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Sunrise tw

Manufactured by Tecan
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Sourced in Switzerland, Austria

The Sunrise TW is a multimode microplate reader designed for various applications in life science research. It provides accurate absorbance measurements for a wide range of wavelengths, enabling researchers to perform diverse assays and analyses.

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Lab products found in correlation

Cryovial, by Greiner (1 mentions) Insulin, by Mercodia (1 mentions) Krc3011, by Thermo Fisher Scientific (1 mentions) Bms625, by Thermo Fisher Scientific (1 mentions) Kge013, by R&D Systems (1 mentions)

12 protocols using sunrise tw

1

Quantification of Thyroid Hormone Levels

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Blood samples were obtained from the heart after sacrifice. The samples were
kept at 5°C until centrifugation at 1000×g. Serum was
distributed into sterile Cryo Vials (Greiner Labortechnik, Frickenhausen,
Germany) in volumes of 500 μL and immediately frozen at
−80°C until analysis. The levels of plasma thyroid hormones,
including triiodothyronine (T3), thyroxine (T4), and thyroid stimulating
hormone (TSH) were measured by enzyme-linked immunosorbent assay according
to the manufacturer’s protocol (Endocrine Technology, Old Bridge, NJ,
USA). The absorbance was measured 450 nm using a plate reader (Tecan Sunrise
TW, Salzburg, Austria).
Kim S.Y., Hong Y.P, & Yang Y.J. (2021). The Impairment of Thyroid Hormones Homeostasis after Short-Term Exposure to Di(2-ethylhexyl)phthalate in Adolescent Male Rats. Development & Reproduction, 25(4), 293-298.
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2

Serum Biomarker Measurement Protocol

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Serum leptin (IBL Japan, Gunma, Japan; 27295), insulin (Mercodia AB, Uppsala, Sweden; 10-1124-01), adiponectin (Adipogen Inc., Incheon, Korea; AG-45A-0005EK-KI01), hepatic interleukin-6 (Thermo Fisher Scientific, Inc., Waltham, MA, USA; BMS625), and tumour necrosis factor-α (Thermo Fisher Scientific, Inc., Waltham, MA, USA; KRC3011) were measured using enzyme-linked immunosorbent assay kits according to the manufacturer’s protocol. The absorbance was measured using a plate reader (Tecan Sunrise TW, Salzburg, Austria).
Lee E.J., Hong Y.P, & Yang Y.J. (2024). Short-term exposure to di(2-ethylhexyl)phthalate may disrupt hepatic lipid metabolism through modulating the oxidative stress in male adolescent rats. Environmental Analysis, Health and Toxicology, 39, e2024007.
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3

Hepatic Triglyceride Quantification

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Frozen liver samples were homogenised in PBS (1:9, w/v) on ice and sonicated to further break the cell membranes. Minced tissues were centrifuged for 5 min (5000 ×g, at 4 °C). The supernatants were collected to measure the hepatic TG levels. The preparation and measurement of TG content in the liver samples were performed according to the manufacturer’s protocol (MyBiosource, Inc., San Diego, CA, USA; MBS164762). Absorbance (450 nm) was measured using a plate reader (Tecan Sunrise TW, Salzburg, Austria).
Lee E.J., Hong Y.P, & Yang Y.J. (2024). Short-term exposure to di(2-ethylhexyl)phthalate may disrupt hepatic lipid metabolism through modulating the oxidative stress in male adolescent rats. Environmental Analysis, Health and Toxicology, 39, e2024007.
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4

Measurement of Malondialdehyde in Liver and Serum

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The frozen liver samples were homogenized in deionized water and minced samples were sonicated for 10 s and frozen/thawed at ≤ - 20 °C (repeated three times). The serum and homogenized liver samples were aliquoted to a microcentrifuge tube (300 μL) and thiobarbituric acid reactive substances acid reagent (300 μL) was added and mixed well. The samples were incubated for 15 min at room temperature and centrifuged for 4 min (≥ 12000 ×g, at 4 °C). The supernatants were collected to measure MDA levels. The preparation and measurement of MDA in the serum and liver were performed according to the manufacturer’s protocol (R&D Systems Inc., Minneapolis, MN, USA; KGE013). The absorbance (532 nm) was measured using a plate reader (Tecan Sunrise TW, Salzburg, Austria).
Lee E.J., Hong Y.P, & Yang Y.J. (2024). Short-term exposure to di(2-ethylhexyl)phthalate may disrupt hepatic lipid metabolism through modulating the oxidative stress in male adolescent rats. Environmental Analysis, Health and Toxicology, 39, e2024007.
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5

Cytotoxicity Evaluation of PA in HDFs

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The cytotoxicity measurement of PA proceeded at a density of 5 × 104 cells/well in a 24-well plate for 24 h, then treated with PA at four different concentrations—0, 0.5, 1, 2 and 3 μg/mL—for another 24 h. The cell viability of HDFs was determined by using a colorimetric MTT assay as described by Ding et al. [39 ]. Briefly, 100 µL of MTT stock solution (2 mg/mL in PBS buffer) was added to each well and then incubated for 3 h. After the incubation, each of the mixture mediums was discarded and 300 µL of 100% DMSO was added to dissolve an insoluble formazan. The absorbance was measured at 540 nm using a microplate reader (Sunrise TW, Tecan Trading AG, Mannedorf, Switzerland).
Ding Y., Jiratchayamaethasakul C, & Lee S.H. (2020). Protocatechuic Aldehyde Attenuates UVA-induced Photoaging in Human Dermal Fibroblast Cells by Suppressing MAPKs/AP-1 and NF-κB Signaling Pathways. International Journal of Molecular Sciences, 21(13), 4619.
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6

Elastase Inhibition Assay Protocol

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Elastase inhibition was determined by measuring the intensity of the solution color assayed using the method of Tu and Tawata (2015) with slight modifications. The mixture of AAAPVN elastase substrate in 0.1232 M Tris-HCl buffer solution (pH 8) was prepared to obtain a concentration of 1.015 mM. The elastase substrate was mixed with the 10 μl of sample in the 96-well plates, and preincubated at 25 °C for 10 min. After the preincubation, the reaction was initiated by adding 10 μl of elastase from porcine pancreas (7.5 units/ml) in Tris solution buffer to the preincubated mixtures. Finally, the absorbance was measured at 410 nm using a microplate reader (Sunrise TW, Tecan Trading AG, Männedorf, Switzerland).
Jiratchayamaethasakul C., Ding Y., Hwang O., Im S., Jang Y., Myung S., Lee J.M., Kim H., Ko S., & Lee S. (2020). In vitro screening of elastase, collagenase, hyaluronidase, and tyrosinase inhibitory and antioxidant activities of 22 halophyte plant extracts for novel cosmeceuticals. Fisheries and aquatic sciences, 23(1).
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7

DPPH Radical Scavenging Assay

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The DPPH scavenging activity was performed as described by Nanjo et al. (1996) . Briefly, DPPH reagent was dissolved in methanol for a solution concentration of 1.5 × 10 -4 M. One hundred microliters of DPPH reagent was mixed with 100 μl sample in 96-well plates. After incubation at room temperature for 30 min, the absorbance was measured 517 nm using a microplate reader (Sunrise TW, Tecan Trading AG, Männedorf, Switzerland).
Jiratchayamaethasakul C., Ding Y., Hwang O., Im S., Jang Y., Myung S., Lee J.M., Kim H., Ko S., & Lee S. (2020). In vitro screening of elastase, collagenase, hyaluronidase, and tyrosinase inhibitory and antioxidant activities of 22 halophyte plant extracts for novel cosmeceuticals. Fisheries and aquatic sciences, 23(1).
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8

Collagenase Inhibitory Activity Assay

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Collagenase inhibitory activity was evaluated based on the method described by Wang et al. (2018) with some modifications. A fixed weight of 1 mg of Azo dyeimpregnated collagen was measured in the test tubes and then the homogenization was proceeded after the addition of an 800 μl of 0.1 M Tris-HCl (pH 7) and a 100 μl of sample into each of test tubes. A 100 μl collagenase (200 units/ml) was immediately mixed into the mixture and incubated at 43 °C for 1 h. Afterward, the test tubes were centrifuged at 3000 rpm for 10 min. The supernatant section of each test tube was transferred into 96-well plates and the absorbance of each supernatant was measured at 550 nm (Sunrise TW, Tecan Trading AG, Männedorf, Switzerland).
Jiratchayamaethasakul C., Ding Y., Hwang O., Im S., Jang Y., Myung S., Lee J.M., Kim H., Ko S., & Lee S. (2020). In vitro screening of elastase, collagenase, hyaluronidase, and tyrosinase inhibitory and antioxidant activities of 22 halophyte plant extracts for novel cosmeceuticals. Fisheries and aquatic sciences, 23(1).
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9

Hydrogen Peroxide Scavenging Assay

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Hydrogen peroxide scavenging activity was examined according to Müller (1985) with slight modification. A 100 μl of 0.1 M PBS buffer (pH 5) was added into a 96well plate. Each 20 μl of sample and 20 mM hydrogen peroxide (H 2 O 2 ) were added to mix with the buffer, and then incubate 37 °C for 5 min. After the incubation, a 30 μl of 1.25 mM ABTS and peroxidase (1 unit/ml) were added to the mixture and then incubated at 37 °C for 10 min. The absorbance was read with a microplate reader at 405 nm (Sunrise TW, Tecan Trading AG, Männedorf, Switzerland).
Jiratchayamaethasakul C., Ding Y., Hwang O., Im S., Jang Y., Myung S., Lee J.M., Kim H., Ko S., & Lee S. (2020). In vitro screening of elastase, collagenase, hyaluronidase, and tyrosinase inhibitory and antioxidant activities of 22 halophyte plant extracts for novel cosmeceuticals. Fisheries and aquatic sciences, 23(1).
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10

Enzymatic Inhibition and Activity Assays

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Determination of the α-amylase inhibitory property was as described by Adefegha et al. [16] and according to the previous studies [17] . Collagenase enzyme activity of 2′-hydroxy-5′-methoxyacetophenone was investigated based on the assay described by Wang et al. [18] with several modifications and conforming to previous studies [19] . The supernatant part of some of the test tube was measured at 550 nm (Tecan Trading AG, Sunrise TW, Switzerland, Männedorf). On the other hand, AR enzyme activity of 2′-hydroxy-5′-methoxyacetophenone was investigated by measuring the reduction of NADPH at 340 nm dl glyceraldehyde as the substrate and according to prior studies [20] .
Wang L., & Zhi Y. (2021). Anti-ovarian cancer and collagenase, α-amylase, and aldose reductase inhibition properties of 2'-hydroxy-5'-methoxyacetophenone with molecular modeling studies. Archives of Medical Science.
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