Mouse anti ha tag

Manufactured by BioLegend

Sourced in United States

The Mouse anti-HA tag is a monoclonal antibody that specifically recognizes the hemagglutinin (HA) epitope tag. It is designed for the detection and purification of HA-tagged proteins in various applications.

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Close spelling variants of this product

Anti-HA (86 citations) Mouse anti-HA (41 citations) HA-tag (14 citations) Mouse anti-HA.11 epitope tag antibody (4 citations)

3 protocols using mouse anti ha tag

Antibody Protocols for Western Blotting

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Primary antibodies used for Western blots are as follows: mouse anti-HA-tag (Convance, MMS-101P through BioLegend, United States), mouse anti-Myc-tag (Santa Cruz, United States, sc-40), mouse anti-β-actin (Santa Cruz, United States, sc-47778; Cell Signaling, United States, #3700), mouse anti-α-tubulin (Cell Signaling, United States, #3873), mouse GAPDH (Santa Cruz, United States, sc-47724), rabbit anti-Pfn1 (Cell Signaling, United States, #3246), rabbit anti-VASP (Bethyl laboratories, United States, A304-769A), rabbit anti-cleaved caspase-7 (Cell Signaling, United States, #8438), rabbit anti-cleaved PARP (Cell signaling, United States, #9541), rabbit anti-GFP (Cell Signaling, United States, #2956). To raise the polyclonal pSer⁷¹-Pfn1 antibody (F5675), a synthetic phospho-Pfn1 peptide harboring pSer⁷¹ [Ac-CLGGQKC(pS)VIRDSL-amide] was conjugated to keyhole limpet hemocyanin and used to immunize rabbits. Antiserum was subjected to double affinity purification using both the antigenic phospho-peptide and the same peptide without the phosphate on Ser⁷¹ (New England Peptide, Inc., United States).

Wang F., Zhu C., Cai S., Boudreau A., Kim S.J., Bissell M, & Shao J. (2021). Ser71 Phosphorylation Inhibits Actin-Binding of Profilin-1 and Its Apoptosis-Sensitizing Activity. Frontiers in Cell and Developmental Biology, 9, 692269.

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Immunoblot Antibody List for MCV Research

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Primary antibodies for immunoblots include: rabbit anti-EZH2 (#5246, Cell Signaling), rabbit anti-EZH1 (#42088, Cell Signaling), mouse anti-E2F-1 (sc-251, Santa Cruz), mouse anti-p53 (sc-126, Santa Cruz), rabbit anti-H3K27me3 (#9733, Cell Signaling), mouse anti-Histone H3 (#14269, Cell Signaling), rabbit anti-PARP (#9532, Cell Signaling), rabbit anti-cleaved PARP (#5625, Cell Signaling), mouse anti-Hsp/Hsc70 (sc-7298, Santa Cruz), mouse anti-GAPDH (sc-47724, Santa Cruz), and mouse anti-HA tag (MMS-101, Biolegend). CM2B4 [31 (link)] and 2T2 [32 ] monoclonal antibodies were used to detect MCV LT and sT proteins. Secondary antibodies for immunoblots include anti-mouse IRDye 680 (Li-COR), anti-mouse IRDye 800 (Li-COR), and anti-rabbit IRDye 800 (Li-COR).

Khattri M., Amako Y., Gibbs J.R., Collura J.L., Arora R., Harold A., Li M.Y., Harms P.W., Ezhkova E, & Shuda M. (2023). Methyltransferase-independent function of enhancer of zeste homologue 2 maintains tumorigenicity induced by human oncogenic papillomavirus and polyomavirus. Tumour Virus Research, 16, 200264.

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Immunofluorescence Analysis of Transfected Cells

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HeLa or PC-3 cells were plated (3x10 4 and 4x10 4 , respectively) and transfected on glass 8-well chamber slides. 24 hrs after transfection, the cells were fixed with 4% formaldehyde (5 min, RT), washed in Tris Buffer Saline pH 7.4 (TBS), permeabilized in 0.2% Triton X-100 in TBS (5 min, RT) and blocked (2 x 15 mins, RT) in a blocking buffer (5% BSA in TBS). The primary antibodies, mouse anti-HA tag (Biolegend; 1/750), rabbit anti-Flag tag (Cell Signalling; 1/1000), were diluted in blocking buffer and incubated 1 h at RT. The secondary antibodies (goat anti-mouse Alexa Fluor 594 and goat anti-rabbit Alexa Fluor 488) were diluted 1:1000 and incubated for 1 h at RT. The slides were mounted with ProLong Gold Antifade Reagent containing DAPI (Invitrogen). Images were acquired using a Leica SP8x confocal microscope. LCP staining was performed by adding the dye 1/1000 after the immunofluorescence procedure.

Claes F., Maritschnegg E., Baets G.D., Siekierska A., Rubio M.S., Ramakers M., Michiels E., Smet F.D., Depreeuw J., Vergote I., Vanderstichele A., Broeck A.V.d., Olbrecht S., Hermans E., Amant F., Lambrechts D., Nilsson K.P.R., Rousseau F., & Schymkowitz J. (2020). The tumor suppressor protein PTEN undergoes amyloid-like aggregation in tumor cells.

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