Twenty-nine animals pretreated with either morphine or saline were assigned to the testing of appetitive motivation for social and sexual rewards, i.e., Saline-male (n = 7), Morphine-male (n = 7), Saline-female (n = 7), Morphine-female (n = 8). The apparatus for the testing was shown in Figure 1A . On the day before the appetitive behavior testing, all the subjects were habituated for 30 min in the open-field arena. On the testing day (withdrawal day 7), after habituation for 10 min in the open-field arena, a male rat or a sexually receptive female rat was placed in the stimulus-cage and the behavior of each subject was videotaped for 1 h by a camera and later analyzed using EthoVision software XT 7.1 (Noldus, The Netherlands). The open-field and stimulus-cage were wiped clean with 0.1% acetic acid in water between subjects to eliminate olfactory cues. As the measures of social interest or sexual incentive motivation, for each subject, the time spent on sniffing the stimulus-cage (sniffing time, i.e., nose-point within the wire-screen) and the time spent in the incentive zone (35 × 20 cm) adjoined to the stimulus-cage (zone time, i.e., center-point in the incentive zone) were automatically collected by the software.
Ethovision software xt 7
Manufactured by Noldus
EthoVision software XT 7.1 is a video tracking system for automated behavioral analysis. It can track and analyze the movements and behaviors of animals in a variety of experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ethovision software xt 7
1
Appetitive Motivation for Social and Sexual Rewards
2
Stress and Drug Effects on Social and Sexual Motivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Thirty-nine and forty male rats were used to test the influence of stress on free-approach behavior for social or sexual rewarding stimuli, respectively. For each reinforcer rats were divided into two treatment (vehicle vs. morphine) and stress groups (control vs. foot-shock) as follow: Saline-Control (n=10 and 12), Morphine-Control (n=9 and 9), Saline-Foot-shock (n=10 and 8), Morphine-Foot-shock (n=10 and 11) for social and sexual stimuli, respectively. On the day before appetitive behavior testing (withdrawal day 13), all rats were habituated for 15 min to the open-field arena. On the test day (withdrawal day 14), after the foot-shock stress was introduced, the rats were allowed to habituate for 10 min to the open-field arena. Then either a male, or a sexually receptive female, rat was placed in the stimulus-cage and the behavior of each test rat was videotaped for 3 hours by a camera and later analyzed using EthoVision software XT 7.1 (Noldus, The Netherlands). The open-field chamber and stimulus-cage were wiped clean with 0.1% acetic acid in water between each subject to eliminate olfactory cues. For each subject the time spent on sniffing the stimulus-cage (sniffing time, i.e., nose-point within the wire-screen) was automatically collected by the software as the index of social or sexual motivation.
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