Mucin (porcine stomach, type III), fetuin (fetal calf serum), Cyanogen bromide, MTT (3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) were procured from Co, St. Louis, USA. Sepharose 4B was obtained from Pharmacia Fine Chemicals, Uppsala, Sweden. Standard protein molecular weight marker was procured from Merck, India. Amicon Ultra ltration centrifugation tubes 10 kDa were procured from Millipore, USA. Human blood samples were collected from German hospital, Dharwad. For cell culture, Dulbecco's Modi ed Eagle's Medium F12 (DMEM F12) and foetal bovine serum (FBS) were obtained from Gibco Invitrogen (Paisley, UK), with Costar 96-well plates obtained from Corning Incorporate (Corning NY, USA). Bovine serum albumin (BSA), Fluorescein isothiocyante (FITC) were obtained from Sigma Chemical Co. (St. Louis MO, USA). ELISA kits for IL-6 (#MBS261259), IL-8(#MBS2886709) were obtained from My Biosources. All other reagents used were of analytical grade. Asialofetuin, Asialomucin were prepared from fetuin as described by Spiro and Bhoyroo [18] . Mucin-Sepharose 4B a nity matrix was prepared by coupling fetuin to cyanogen bromide activated Sepharose 4B, according to the method of March et.al [19] .
Amicon ultra ltration centrifugation tubes 10 kda
Manufactured by Merck Group
Sourced in United States
The Amicon Ultra filtration centrifugation tubes 10 kDa are a type of laboratory equipment used for sample concentration and buffer exchange. The tubes are designed to separate molecules based on their molecular weight, with a 10 kDa molecular weight cutoff. This allows for the concentration of samples and the removal of small molecules or salts from the sample.
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2 protocols using amicon ultra ltration centrifugation tubes 10 kda
1
Mucin-Sepharose 4B Affinity Matrix
2
Mucin-Sepharose 4B Affinity Matrix
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Mucin (porcine stomach, type III), fetuin (fetal calf serum), Cyanogen bromide, MTT (3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) were procured from Co, St. Louis, USA. Sepharose 4B was obtained from Pharmacia Fine Chemicals, Uppsala, Sweden. Standard protein molecular weight marker was procured from Merck, India. Amicon Ultra ltration centrifugation tubes 10 kDa were procured from Millipore, USA. Human blood samples were collected from German hospital, Dharwad. For cell culture, Dulbecco's Modi ed Eagle's Medium F12 (DMEM F12) and foetal bovine serum (FBS) were obtained from Gibco Invitrogen (Paisley, UK), with Costar 96-well plates obtained from Corning Incorporate (Corning NY, USA). Bovine serum albumin (BSA), Fluorescein isothiocyante (FITC) were obtained from Sigma Chemical Co. (St. Louis MO, USA). ELISA kits for IL-6 (#MBS261259), IL-8(#MBS2886709) were obtained from My Biosources. All other reagents used were of analytical grade. Asialofetuin, Asialomucin were prepared from fetuin as described by Spiro and Bhoyroo [18] . Mucin-Sepharose 4B a nity matrix was prepared by coupling fetuin to cyanogen bromide activated Sepharose 4B, according to the method of March et.al [19] .
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