Chlorhexidine diacetate cda

The solutions that were to be coated on the orthodontic elastomerics were prepared by combining polymers that are capable of controlling the release of the antimicrobial agents, chlorhexidine using two selected solvents (Table 1). In this research, we used ethyl cellulose (EC; SIGMA ALDRICH, Inc., St. Louis, MO, USA) as the polymer; ethyl cellulose has been proven to be safe and efficacious for use in DDSs in the oral cavity. We used ethanol (EtOH; SIGMA ALDRICH, Inc., St. Louis, Missouri, USA) and dichloromethane (DCM; SIGMA ALDRICH, Inc.) as the polymer solvents due to their safeness and solubility. We dissolved ethyl cellulose completely in the prepared solvent to make a coating solution and added chlorhexidine diacetate (CDA; SIGMA ALDRICH, Inc.) as antimicrobial agents using a stirrer 12) .

Chlorhexidine diacetate (CDA; SIGMA-ALDRICH, St. Louis, MO, USA) was used as the antimicrobial agent, and ethyl cellulose (EC; N100, SIGMA-ALDRICH) was used as the polymer for preparing the coating solution. The volume of polymers used was 2% of that of the solvents, and five study groups were designated, comprising a group with mixtures without polymers as the control, and other groups with polymers and various solvent combinations assigned as the experiment groups (Table 1). In attempts to ensure chemical stability and solubility, ethanol (EtOH; DUKSAN Pure Chemicals, Ansan, Republic of Korea) and dichloromethane (DCM; DUKSAN Pure Chemicals) were used as solvents when varying the ratios of dissolved powdered CDA and EC. EC was dissolved completely in the prepared solvent using a stirrer, and then the antimicrobial agent (CDA) was added to produce the coating solution.