An Agilent 6545 UPLC-QToF run in both positive and negative modes was used for analysis. Separation was achieved using a Sequant Zic-pHILIC 2.1 × 100 mm column (Millipore Sigma) with Phenomenex Krudkatcher (Phenomenex). An initial concentration of 99% ACN with 5% ddH2O (buffer B) and 5% 25 mM ammonium carbonate in ddH2O (buffer B) was held for 1 min at a flow rate of 0.2. B was decreased to 10% over 14 min and held for 5 min. B was returned to starting conditions over 0.1 min, and the system was allowed to re-equilibrate for 10 min between runs at a 0.2 mL/min flow rate.
Sequant zic philic 2.1 100 mm column
Manufactured by Merck Group
The Sequant Zic-pHILIC 2.1 × 100 mm column is a laboratory instrument used for liquid chromatography. It has a zwitterionic stationary phase and is designed for polar compound separations.
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Lab products found in correlation
2 protocols using sequant zic philic 2.1 100 mm column
1
UPLC-QToF Analysis of Metabolites
2
UPLC-QToF Analysis of Metabolites
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An Agilent 6545 UPLC-QToF run in both positive and negative modes was used for analysis. Separation was achieved using a Sequant Zic-pHILIC 2.1 × 100 mm column (Millipore Sigma) with Phenomenex Krudkatcher (Phenomenex). An initial concentration of 99% ACN with 5% ddH2O (buffer B) and 5% 25 mM ammonium carbonate in ddH2O (buffer B) was held for 1 min at a flow rate of 0.2. B was decreased to 10% over 14 min and held for 5 min. B was returned to starting conditions over 0.1 min, and the system was allowed to re-equilibrate for 10 min between runs at a 0.2 mL/min flow rate.
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