Mice were anesthetized and perfused with saline and 4% paraformaldehyde 7 days after MCAO. Apoptosis was analyzed using a TUNEL (terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling) assay kit (Promega, Tokyo, Japan) according to the manufacturer’s instructions and as described previously.7 We randomly selected and photographed 3 areas of the peri‐infarct region (upper, middle, and bottom of the striatum) under a microscope (BZ‐X710; Keyence, Osaka, Japan). Images were collected using imaging software (BZ‐X analysis application; Keyence). All images were processed using the ImageJ software analysis system in a masked manner for unbiased counting, as described in a previous report.7 Mean TUNEL‐positive stained cell counts were calculated for the 3 microscopic fields in the peri‐infarct regions of each section, and sections were analyzed for each brain. Data are shown as the mean number of cells per square millimeter.
Bz x analysis application
Manufactured by Keyence
The BZ-X analysis application is a software tool that enables the analysis and processing of images captured using Keyence's BZ-X Series microscopes. It provides users with the ability to perform various image analysis tasks, such as object counting, measurement, and classification. The core function of the BZ-X analysis application is to facilitate the analysis and interpretation of microscopic images.
Automatically generated - may contain errors
4 protocols using bz x analysis application
1
Quantifying Apoptosis in Ischemic Stroke
2
Immunofluorescent Staining of Tumor Spheres
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tumour spheres were fixed with 4% paraformaldehyde containing 0.2% Triton X-100 for 20 min and then incubated with 25% BlockAce (KAC Co. Ltd, Tokyo Japan) for 10 min. Anti-ALDH1A1 antibody (×500 dilution in BlockAce) was used as the first antibody and AF488-labeled anti-mouse IgG was used as the second antibody. Stained spheres were observed with a fluorescent microscope (BIOREVO BZ-9000, Keyence, Osaka, Japan). The average fluorescent intensity was obtained by BZ-X Analysis Application (Keyence).
3
Immunohistochemical Analysis of Tumor Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sections (3 μm) of paraffin‐embedded tumor samples were used for immunohistochemical analysis. The slides were heated for antigen retrieval in 10 mM sodium citrate (pH 6.0). Sections were subsequently exposed to specific antibodies for TUNEL (#MK500; Takara Bio Inc.) or Ki‐67 (#M7240; DAKO). These were then incubated with Histofine Simple Stain MAX‐PO (MULTI) (Nichirei Biosciences Inc.). Staining was revealed using diaminobenzidine (Nichirei Biosciences Inc.) and the slides were counterstained with aqueous hematoxylin. After counterstaining the cells, the slides were analyzed under a microscope (BZ‐X700; KEYENCE). Then, immunostaining‐positive cells were counted and quantified using the BZ‐X analysis application (KEYENCE).
4
Histological Analysis of Muscle Fibrosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For histological analysis, TA muscles were excised, fixed in 10% neutralized formalin, and embedded in paraffin. Serial sections at 5 μm were deparaffinized and stained with hematoxylin and eosin for morphological analysis and Masson’s trichrome for evaluation of fibrosis. Images were acquired with a microscope (FSX100; Olympus), and for measurement of the cross-sectional area of centronuclear myofibers, images of random fields of injured area were analyzed using WinROOF software (Mitani Corporation). We evaluated a ratio of fibrotic area to injured area for quantification of the fibrotic area (%) in images acquired with a microscope (BZ-X700; Keyence Corporation) using BZ-X analysis application (Keyence Corporation).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!