A2780

The OVCAR-8 (NCI-DTP Cat# OVCAR-8) were obtained from the NCI-DCTD repository. The A2780 (NCI-DTP Cat# A2780) ovarian carcinoma cells lines and the LNCaP prostate carcinoma cell line (CLS Cat# 300265/p761_LNCaP) were purchased from American Type Culture Collection (ATCC,). Cell cultures were maintained following the protocols suggested by the providers.

The human ovarian cancer cell lines, OVCAR-4, OVCAR-8, A2780, cisplatin-resistant A2780 (A2780cis) were bought from American Type Culture Collection (Manassas, VA, USA), and human ovarian epithelial (HOE) cells immortalized using SV40 large T antigen (Catalogue number: T1074) were purchased from Applied Biological Materials Inc. (Richmond, BC, Canada). All cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% fetal bovine serum (FBS), penicillin-streptomycin (50 U/ml) and glutamine (2 mM).

Expression constructs encoding for TET1 or TET3 C-terminal cysteine-rich dioxygenase (CD) domain (Flag-TET1 or Flag-TET3) and activity-dead mutant TET1 (TET1-MU) or TET3 (TET3-MU) were kindly provided by Dr. Heng-yu Fan.
Human ovarian cancer cell lines, SKOV3, A2780, ES-2, HO8910, OV2008, C13, 293T, and Hep3132 were purchased from ATCC. The immortalized mouse ovarian surface epithelium (mOSE) were reported previously [41 (link)]. All cell lines were cultured under an atmosphere of 5% CO₂ at 37°C. All cells were cultured in Dulbecco’s Modified Eagle Media( DMED, Gibco) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin-streptomycin solution (Gibco). The chemotherapeutic drugs used in this study included paclitaxel (0.01 μg/mL) (Bristol-Myers Squibb, New York, NY, USA). MLN4924 (MedChemExpress) was applied in the concentration of 1 μM. All reagents were used according to the instructions provided by the suppliers.

OVCAR3, A2780, 3AO, and SKOV-3 (ovarian tumor cell lines) and IOSE80 (normal ovarian cell line) were purchased from ATCC of the United States. OVCAR3, 3AO, and A2780 were cultured in RPMI 1640 medium supplemented with penicillin (100 U/mL), streptomycin (100 mg/L), and 10% v/v fetal bovine serum (FBS). IOSE80 and SKOV3 were cultured in DMEM and McCoy's 5A medium containing penicillin (100 U/mL), streptomycin (100 mg/L), and 10% FBS, respectively. The cells were cultured at 37°C and 5% CO₂. (The culture medium and supplements were purchased from Invitrogen.)

Human umbilical artery endothelial cells (HUAECs) and human umbilical vein endothelial cells (HUVECs) were purchased from Lonza Walkersville, MD, USA, and maintained in corresponding medium provided by Lonza using University of Southern California Institutional Review Board approved tissue procurement protocol. HEK293T, H211, A2780, HOC7, MCF7, MDA-MB231, HT29, SCC15, Colo205, Hela and 211H cell lines were from ATCC. Human Tspan18 tagged with Myc was cloned into pcDNA3.1 (Invitrogen, Carlsbad, CA, USA). Mouse VEGFR2 in pCMV-SPORT6 was from Open Biosystems (Huntsville, AL, USA, Cat# MMM1013-65680). Transfection of HEK293T cells was performed with BioT (Bioland Scientific, Cerritos, CA, USA) according to the manufacturer's protocol.