Caov4

Human OC cell lines (SKOV3, OVCAR3, and Caov-4), human normal ovarian epithelial cell line (IOSE80), and 293T cell line were obtained from ATCC (USA). Another human healthy ovarian cell line (HOSEpiC) was purchased from ScienCell Research Laboratories (Cat#: 7310, USA). SKOV3 cells were cultured in McCoy’s 5A medium (Gibco, USA) with 10% (v/v) fetal bovine serum (FBS, Invitrogen, USA). Caov-4, IOSE-80, and 293T cell lines were cultured in DMEM medium (HyClone, USA) and supplemented with 10% (v/v) FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. HOSEpiC cell line and OVCAR3 cell line were cultured in RPMI-1640 medium (HyClone, USA) and augmented with 10% (v/v) FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. All the cells were placed in an atmosphere of 5% CO₂ at 37 °C.

SKOV3 (Cat: HTB-7) cells were purchased from American Type Culture Collection (ATCC) and cultured in ATCC-formulated McCoy's 5a Medium Modified (Cat: 30-2007) plus 10% fetal bovine serum (FBS, Gibco, Rockville, USA) and 50 μg/ml penicillin/streptomycin (P/S, Gibco, Rockville, USA). CAOV4 (Cat: HTB-76) cells also were purchased from ATCC but cultured in ATCC-formulated Leibovitz's L-15 Medium (Cat: 30-2008) plus 10% FBS and 50 μg/ml P/S. A2780 (Cat: 93112519) cells were purchased from the European Collection of Authenticated Cell Cultures (ECACC) and cultured in RPMI-1640 plus 2 mM Glutamine, 10% FBS, and 50 μg/ml P/S. JHOS4 (Cat: RCB1678) cells were purchased from cell bank of RIKEN BioResource Research Center and cultured in DMEM/HamF12 plus 0.1 mM NEAA, 10% FBS, and 50 μg/ml P/S. EFO21 (Cat: ACC235) was purchased from DSMZ-German Collection of Microorganisms and Cell Cultures and cultured in RPMI-1640 plus 10% FBS, 50 μg/ml P/S, 2 mM glutamine, 1 × MEM nonessential amino acids, and 1 mM sodium pyruvate. OVCAR4 cells were purchased from COBIOER Company (Nanjing, China) and cultured in RPMI-1640 supplemented with 10% FBS plus 50 μg/ml P/S. All the cells were maintained in a CO₂ incubator (with inner 37°C).