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2 protocols using oleuropein

1

Quantitative Analysis of Phenolic Compounds

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Standards of the following phenolic compounds were obtained from PhytoLab GmbH & Co. KG (Vestenbergsgreuth, Germany): apigenin, apigenin-7-O-glucoside, trans-cinnamic acid, p-coumaric acid, trans-ferulic acid, protocatechuic acid, maslinic acid, vanillic acid, tyrosol, hydroxytyrosol, hydroxybenzoic acid, hydroxytyrosol acetat, oleanolic acid, oleoside-11-methylester, luteolin, luteolin-7-O-glucoside, (+)-pinoresinol, 1-acetoxypinoresinol, oleuropein, oleacein, p-HPEA-EA, and oleocanthal. Acetonitrile, methanol, water, and formic acid (LC-MS grade) were purchased from Sigma Aldrich Chemie GmbH (Buchs, Switzerland). 2 M Folin-Ciocalteau reagent and anhydrous sodium carbonate (both from Sigma) were used to measure the TPC.
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2

Bioactive Compounds Sourcing and Analysis

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Hydroxytyrosol, tyrosol, gallic acid, fraxetin, oleuropein, verbascoside, catechin, luteolin 3,7 glucoside, procyanidin B1, quercitin 3-O-glucoside, and elenoic acid were obtained from PhytoLab GmbH & Co. (Vestenbergsgreuth Germany). fraxetin was from Sigma Chemical Co (St Louis, MO). Fetal bovine serum (FBS) was from Hyclone Perbio Sciences (Helsingborg, Skane Lan, Sweden). Human interleukin (IL)-1β, IL-6 and IL-8 enzyme-linked immunosorbent assay (ELISA) Kits II were from BD Biosciences Pharmingen (San Jose, CA, USA). HPLC-grade solvents and all other reagents and chemicals were from Sigma Chemical Co (St. Louis, MO, USA).
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