Cell lines were sourced and cultured as described previously (12 (link),18 (link),26 (link)). A673 (ATCC Cat# CRL-1598, RRID: CVCL_0080) and SK-N-MC (ATCC Cat# CRL-2270, RRID: CVCL_1398) cell lines were obtained from ATCC, TC-32 from Dr. Timothy Triche (Children’s Hospital of Los Angeles), and SK-N-MC from Dr. Shu-Fang Jia (University of Texas, Houston, TX). Cell lines were validated yearly using STR profiling. Cell lines were tested for the presence of Mycoplasma using the Universal Mycoplasma testing kit every 3–4 months as needed and were most recently tested in December 2021. Cells that were freshly thawed were used up to two months in culture. Monoclonal cell lines generated in this study were expanded and stored long-term in 90% Fetal Bovine Serum (FBS) and 10% DMSO in liquid nitrogen. Soft agar assays were performed as described (12 (link),18 (link),26 (link)). SP-2509 was purchased from Cayman Chemicals. Antimycin A, rotenone, and oligomycin were purchased from Thermo-Fisher. All oligonucleotides used in this study were purchased from Integrate DNA Technologies (IDT), or Thermo-Fisher Scientific.
Sk n mc
Manufactured by American Type Culture Collection
Sourced in United States, Germany, France
SK-N-MC is a human cell line derived from a neuroblastoma. The cell line is adherent and exhibits an epithelial-like morphology. SK-N-MC cells are commonly used for research purposes in the fields of neuroscience, oncology, and cell biology.
Automatically generated - may contain errors
Lab products found in correlation
Rd es, by American Type Culture Collection (15 mentions)
Sk es 1, by American Type Culture Collection (10 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions)
Imr 32, by American Type Culture Collection (6 mentions)
Sh sy5y, by American Type Culture Collection (5 mentions)
Sk n sh, by American Type Culture Collection (5 mentions)
Sk n mc, by Thermo Fisher Scientific (4 mentions)
Penicillin, by Thermo Fisher Scientific (4 mentions)
Streptomycin, by Thermo Fisher Scientific (4 mentions)
Tc 32, by American Type Culture Collection (3 mentions)
Fetal bovine serum (fbs), by Merck Group (3 mentions)
Htb 166, by American Type Culture Collection (3 mentions)
Mycoalert mycoplasma detection kit, by Lonza (3 mentions)
Tc 71, by Leibniz Institute DSMZ (3 mentions)
Sk n as, by American Type Culture Collection (3 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions)
Antibiotic antimycotic solution, by Merck Group (2 mentions)
Umuc3, by American Type Culture Collection (2 mentions)
Human platelet lysate, by STEMCELL (2 mentions)
Human serum, by Corning (2 mentions)
44 protocols using sk n mc
1
Cell Line Sourcing and Validation
2
Culture of Ewing's Sarcoma Cell Lines
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Bone metastatic Ewing’s sarcoma cell line SK-N-MC (HTB-10) and primary Ewing’s sarcoma cell line RD-ES (HTB-166) were purchased from the American Type Culture Collection (ATCC) and cultured according to the manufacturer’s specifications. RD-ES cells were cultured in ATCC-formulated RPMI-1640 Medium (RPMI) and SK-N-MC cells were cultured in ATCC-formulated Eagle’s Minimum Essential Medium (EMEM). Both media were supplemented with 1% penicillin/streptomycin, and either 10% (v/v) Hyclone FBS, 10% (v/v) human serum (Corning, 35-060), or 5% (v/v) Human Platelet Lysate (Stemcell, 6961). Cells were cultured at 37°C in a humidified incubator at 5% CO2.
3
Cultivation of Human and Monkey Cell Lines for Viral Research
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Human neuroepithelioma cell line SK-N-MC (ATTC HTB-10, ATCC, Manassas, Virginia, USA), and African green monkey kidney Vero cell line (ATTC CCL-81, ATCC, Manassas, Virginia, USA) were obtained from the American Type Culture Collection. Stable transfected SK-N-MC amyloid precursor protein (SK-APP-D1, these cells were generated from SK-N-MC cells (ATCC HTB10, Manassas, Virginia, USA) modified to stably expressing the human isoform APP695 and these cells were kindly provided by Dra. MJ. Bullido (Centro Biología Molecular Severo Ochoa, CBMSO, Madrid, Spain).
SK-N-MC and SK-N-MC APP-D1 cell lines were grown in Dulbecco’s Modified Eagle’s Medium (DMEM; Biochrom AG, Berlin, Germany) supplemented with 10% fetal bovine serum (FBS); Vero cell line was grown in DMEM supplemented with 5% FBS. All culture mediums contain 1% L-glutamine, and an antibiotic mix (125 μg/mL ampicillin, 125 μg/mL cloxacillin and 40 μg/mL gentamicin); (Sigma, St. Louis, Missouri, USA). All cell lines were cultivated in 5% CO2 at 37 °C.
HSV-1 strain Kos 1.1 was kindly provided by Dra. MJ. Bullido and expanded on the Vero cell line, titrated by plaque assay and stored at −80 °C.
SK-N-MC and SK-N-MC APP-D1 cell lines were grown in Dulbecco’s Modified Eagle’s Medium (DMEM; Biochrom AG, Berlin, Germany) supplemented with 10% fetal bovine serum (FBS); Vero cell line was grown in DMEM supplemented with 5% FBS. All culture mediums contain 1% L-glutamine, and an antibiotic mix (125 μg/mL ampicillin, 125 μg/mL cloxacillin and 40 μg/mL gentamicin); (Sigma, St. Louis, Missouri, USA). All cell lines were cultivated in 5% CO2 at 37 °C.
HSV-1 strain Kos 1.1 was kindly provided by Dra. MJ. Bullido and expanded on the Vero cell line, titrated by plaque assay and stored at −80 °C.
4
Neuroblastoma Cell Line Oxidative Stress
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The neuroblastoma cell line (SK-N-MC) was purchased from ATCC (Manassas, VA, USA). The cell culture media of glucose free DMEM and dialyzed FBS were obtained from Invitrogen (Rockville, MD, USA). The reagents including carnosine, DCF-DA (2',7'-dichlorodihydrofluorescein diacetate), TTC (2,3,5-triphenyltetrazolium chloride) and antimycin A were obtained from SIGMA-ALDRICH (St. Louis, MO, USA). Antibodies of superoxide dismutase (SOD)-1, growth associated protein (GAP) 43 and GPADH were used from Cell Signalling Technology Inc. (Danvers, MA, USA). All commercial ELISA kits including SOD, CAT (catalase) and GPx (glutathione peroxidase) were purchased from Biovision (Milpitas, CA, USA), Cell Biolabs Inc. (San Diego, CA, USA) and Dojindo (Kumamoto, Japan).
5
Cell Line Culture Protocols
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HEK293, SH-SY5Y, IMR32, SK-N-AS, and KELLY cell lines were purchased from HPA Culture Collections (England, UK), SK-N-MC and PFSK1 were purchased from ATCC (Virginia, USA). The EBV transformed lymphoblast cell lines GM22671 and GM22737 were obtained from Coriell Cell Repositories (NJ, USA). All cell lines were cultured at 5% CO2 and 37°C in the appropriate culture media. Adherent (non-EBV) cell lines were supplemented with 10% fetal bovine serum (Invitrogen, CA, USA) and 1% penicillin/streptavidin (Invitrogen). EBV cell lines were supplemented with 15% fetal bovine serum (Invitrogen) and 1% penicillin/streptavidin (Invitrogen). The culture medium used to grow HEK293 cells was DMEM (Invitrogen), SK-N-MC and IMR32 were grown in MEM (Invitrogen) supplemented with 2 mM L -glutamine (Invitrogen), and SH-SY5Y and SK-N-AS were grown in DMEM:F12 (Invitrogen) supplemented with 2 mM L -glutamine and 1% non-essential amino acids (Invitrogen). KELLY, PFSK1, GM22671, and GM22737 were grown in RPMI 1640 media (Sigma-Aldrich, MO, USA) supplemented with 2 mM glutamine.
6
Cell Line Culturing Protocol for Sarcoma Research
7
Neuroblastoma Cell Culture Protocol
8
Culturing Primary Neuroglial Cells
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In this study, we used immortalized CHME-5 microglial cells, primary astrocytes, BMVC and SK-N-MC neuroblastoma cell lines. Primary cells astrocytes and BMVC were maintained in basal medium containing 10% fetal bovine serum, 50 units/ml of penicillin, with growth supplement, and 100 μg/ml of streptomycin (Sciencell, Carlsbad,CA). The neuroblastoma cell line, SK-N-MC was purchased from ATCC (catalog # HTB-10;Manassas, VA) and the immortalized CHME-5 microglial cells were cultured in Eagle’s minimum essential medium (MEM) supplemented with fetal bovine serum to a final concentration of 10% and 1% antibiotic ⁄ antimycotic solution (Sigma-Aldrich, St. Louis, MO).
9
Diverse Cell Lines with STAG2 Perturbations
10
Cell Culture Protocols for ENCODE
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