Bt 474 htb 20

Manufactured by American Type Culture Collection

Sourced in United States

The BT-474 (#HTB-20) is a cell line derived from a human breast carcinoma. It is available for purchase from the American Type Culture Collection (ATCC) product catalog.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Rosiglitazone, by Santa Cruz Biotechnology (1 mentions) Donepezil, by Santa Cruz Biotechnology (1 mentions) Ketotifen, by Merck Group (1 mentions) Genistein, by Merck Group (1 mentions) Crizotinib, by Selleck Chemicals (1 mentions) Charcoal stripped fetal bovine serum, by Merck Group (1 mentions) Mts cell proliferation assay, by Promega (1 mentions) Verapamil, by Merck Group (1 mentions) Mda mb 231 htb 26, by American Type Culture Collection (1 mentions) 4t1 murine breast cancer, by American Type Culture Collection (1 mentions) Lapatinib, by GlaxoSmithKline (1 mentions) Sk br 3, by Lonza (1 mentions) Bt474, by Lonza (1 mentions) Rpmi 1640 medium with l glutamine, by Lonza (1 mentions) Mccoy s 5a medium, by Lonza (1 mentions) Skbr3 htb 30, by American Type Culture Collection (1 mentions) Ecacc85090402, by European Collection of Authenticated Cell Cultures (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)

7 protocols using bt 474 htb 20

Anticancer Compound Screening Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fulvestrant and sorafenib were purchased from AK Scientific (Mountain
View, CA); crizotinib from Selleck Chemicals (Houston, TX); rosmarinic
acid, donepezil, and rosiglitazone from Santa Cruz Biotechnology (Dallas,
TX); ketotifen, verapamil, genistein, and GLP-1R agonist from Sigma-Aldrich
(St. Louis, MO); benzoquinoline from Spectrum Chemicals (New Brunswick,
NJ); AFP 07 from Caymen Chemical (Ann Arbor, MI); phenol red from
Amresco (Solon, OH); tetrahydroberberine from Vitas-M (Moscow, Russia).
Ultrapure polysorbate 80 (UP 80) was purchased from NOF Corporation
(White Plains, NY). Dulbecco’s phosphate buffered saline (DPBS)
and RPMI 1640 cell culture media were purchased from Multicell Technologies
(Woonsocket, RI). Charcoal-stripped Fetal bovine serum (FBS) was purchased
from Sigma-Aldrich (St. Louis, MO). Cell lines MDA-MB-231 (HTB-26),
MCF-7 (HTB-22), SK-BR-3 (HTB-30), and BT-474 (HTB-20) were purchased
from ATCC (Manassas, VI). MTS cell proliferation assay was purchased
from Promega (Madison, WI). Duke Standards NIST Traceable Polymer
Microspheres were purchased from Thermo Scientific. FluoSpheres fluorescent
nanoparticles were purchased form Life Technologies (Burlington, ON).
All other chemicals and reagents were purchased from Sigma-Aldrich
(St. Louis, MO) or TCI America (Portland, OR).

Owen S.C., Doak A.K., Ganesh A.N., Nedyalkova L., McLaughlin C.K., Shoichet B.K, & Shoichet M.S. (2014). Colloidal Drug Formulations Can Explain “Bell-Shaped” Concentration–Response Curves. ACS Chemical Biology, 9(3), 777-784.

+ Open protocol

+ Expand

Comprehensive Breast Cancer Cell Line Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human normal breast epithelial cell line (MCF-10A, CL-0525) and BC cell lines (MDA-MB-231 (CL-0150), SK-BR-3 (SKBR-3, CL-0211), MCF-7 (CL-0149), HS-578T (CL-0114), MDA-MB-468 (CL-0290), T-47D (CL-0228), and BT-474 (CL-0040)) were obtained from Procell (Wuhan, China); BC cell lines (HCC1806 (CRL-2335) and BT-474 (HTB-20)) were acquired from ATCC (Rockville, USA) and cultivated in the recommended media. All cell lines were subcultured in our laboratory for less than six months.

Yu J., Zhang X., He R., Yang X., Hu J., Tan F., Yao J., Lei X, & Wen G. (2022). LINC01234 Accelerates the Progression of Breast Cancer via the miR-525-5p/Cold Shock Domain-Containing E1 Axis. Disease Markers, 2022, 6899777.

+ Open protocol

+ Expand

Cell Culture Protocols for Cancer Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Murine breast cancer 4T1 (#CRL-2539) and LLC1 (#CRL-1642), colon cancer (CT26) (#CRL-2638), and human MCF-7 (#HTB-22), BT-474 (#HTB-20), and MDA-MB-231 (#HTB-26) breast cancer cells were purchased from American Type Culture Collection, USA. 4T1-12B cells were a gift from G. Sahagian, Tufts University, USA. 4T1 and 4T12B cells were cultured in RPMI-1640, and LLC1, MCF-7, MDA-MB-231, and BT-474 cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (FBS) and 1× penicillin-streptomycin solution at 37°C and 5% CO₂. Cells were grown to 70 to 80% confluency and harvested using 1× trypsin.

Kar A., Jain D., Kumar S., Rajput K., Pal S., Rana K., Kar R., Jha S.K., Medatwal N., Yavvari P.S., Pandey N., Mehta D., Sharma H., Bhattacharya D., Pradhan M.K., Sharma R.D., Srivastava A., Agrawal U., Mukhopadhyay A., Sengupta S., Patil V.S., Bajaj A, & Dasgupta U. (2023). A localized hydrogel-mediated chemotherapy causes immunogenic cell death via activation of ceramide-mediated unfolded protein response. Science Advances, 9(26), eadf2746.

+ Open protocol

+ Expand

Generating Lapatinib-Resistant Breast Cancer Cell Lines

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human SkBr3 (HTB‐30) and BT474 (HTB‐20) breast cancer cell lines were obtained from the American Type Culture Collection (ATCC). Lapatinib‐resistant (LR) clones were generated from long‐term treatment with Lapatinib (GlaxoSmithKline).²⁴ (link), ²⁵ (link) All cell lines were cultured in Dulbecco's modified Eagle medium/F12 (SH30004.04, HyClone) supplemented with 10% fetal bovine serum (10437‐028, Gibco), 1% penicillin‐streptomycin (SH40003.01, HyClone) at 37°C in an humidified atmosphere of 95% air and 5% CO₂. LR clones were maintained in the presence of 1 μmol/L Lapatinib.

Huynh T.K., Huang C., Chen J., Yao J., Yang Y., Wei Y., Chen H., Chen C., Tu C., Hsu Y., Liu L, & Huang W. (2021). miR‐221 confers lapatinib resistance by negatively regulating p27kip1 in HER2‐positive breast cancer. Cancer Science, 112(10), 4234-4245.

+ Open protocol

+ Expand

Characterization of HER2+ Breast Cancer Cell Lines

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

HER2+ breast cancer cell lines BT474 (HTB-20) representing Luminal B (ER+; PR+, HER2+; ductal carcinoma) subtype and SKBR3 (HTB-30) representing HER2+ subtype (ER-; PR-; HER2+; adenocarcinoma) of breast cancer were purchased from the American Type Culture Collection (ATCC) [25 (link),26 (link)], which were known to well characterized in previous studies and decribed highly sensitive to trastuzumab treatment [27 (link),28 ]. SKBR3 cells were maintained in Mc Coy’s 5A medium (Lonza) with L-glutamine containing 10% fetal bovine serum (FBS), 1% penicillin-streptomycin. BT474 cells were maintained in RPMI 1640 medium with L-glutamine (Lonza) supplemented with 10% FBS, 1% penicillin-streptomycin and 2% bovine insulin. The cell lines were cultured in a humidified air supplemented with 5% CO₂ at 37°C. Trastuzumab was (kindly gifted by Prof. Dr. Hakan Gürdal from the Department of Pharmacology in Medical School of Ankara University) dissolved in phosphate-buffered saline (PBS) (stock concentration of 300 mg/mL) and stored at 4°C.

Cilek E.E., Ozturk H, & Gur Dedeoglu B. (2017). Construction of miRNA-miRNA networks revealing the complexity of miRNA-mediated mechanisms in trastuzumab treated breast cancer cell lines. PLoS ONE, 12(10), e0185558.

+ Open protocol

+ Expand

Culturing Human and Mouse Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human epidermis carcinoma cell line A431 (ECACC85090402) was obtained from the European Collection of Authenticated Cell Cultures through DS Pharma Biomedical (Osaka, Japan). The mouse embryo fibroblast cell line BALB/3T3 clone A31 (JCRB9005) was obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). The human breast ductal carcinoma cell line BT-474 (HTB-20) was obtained from the American Type Culture Collection through Summit Pharmaceuticals International Corporation (Tokyo, Japan). These cell lines were cultured in Dulbecco’s modified Eagle’s medium (10569010; Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (26140079; Thermo Fisher Scientific) and 1% penicillin/streptomycin (15140122; Thermo Fisher Scientific). Each cell line was cultured in tissue culture flasks at 37 °C in a 5% CO₂ humidified atmosphere.

Yamashita S., Kojima M., Onda N, & Shibutani M. (2023). In Vitro Comparative Study of Near-Infrared Photoimmunotherapy and Photodynamic Therapy. Cancers, 15(13), 3400.

+ Open protocol

+ Expand

Breast Cancer Cell Line Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Breast cancer cell lines MCF-7 (TCHu74), MDA-MB-231 (TCHu104) were purchased from the Shanghai Cell Bank, Chinese Academy of Sciences. BT474 (HTB-20™), and T-47D (HTB-133) and normal mammary epithelial cell line MCF 10A (CRL-10317) were obtained from the American Type Culture Collection (Manassas, VA, USA). The cell identity was confirmed by STR analysis. The MCF-7, BT474, MDA-MB-231 and T-47D cells were cultured in Dulbecco's modified Eagle's medium (DMEM; HyClone; GE Healthcare Life Sciences, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS) (HyClone; GE Healthcare Life Sciences). The MCF-10A cells were cultured in DMEM/F12 medium (HyClone; GE Healthcare Life Sciences) containing 5% horse serum (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 1% penicillin/streptomycin (HyClone; GE Healthcare Life Sciences), 20 ng/ml of EGF (Gibco; Thermo Fisher Scientific, Inc.), 0.5 µg/ml of hydrocortisone (Sigma, St. Louis, MO, USA). All cells were incubated in a humidified atmosphere with 5% CO₂ and humidified sphere of 95% at 37°C.

Wang Y., Sui Y., Zhu Q, & Sui X. (2017). Hsa-miR-599 suppresses the migration and invasion by targeting BRD4 in breast cancer. Oncology Letters, 14(3), 3455-3462.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!