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Plc prf 5

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The PLC/PRF/5 is a human hepatocellular carcinoma cell line. It is a well-established and widely used in vitro model for the study of liver cancer and related research.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (27 mentions) Hepg2, by National Collection of Authenticated Cell Cultures (26 mentions) Hep3b, by National Collection of Authenticated Cell Cultures (23 mentions) Smmc 7721, by National Collection of Authenticated Cell Cultures (19 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (15 mentions) Sk hep1, by National Collection of Authenticated Cell Cultures (12 mentions) Mhcc 97h, by National Collection of Authenticated Cell Cultures (7 mentions) Hcclm3, by National Collection of Authenticated Cell Cultures (7 mentions) Plc prf 5, by Thermo Fisher Scientific (7 mentions) Bel 7402, by National Collection of Authenticated Cell Cultures (6 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (4 mentions) Hep3b, by Thermo Fisher Scientific (4 mentions) Hl 7702, by National Collection of Authenticated Cell Cultures (4 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (4 mentions) Hepg2, by Thermo Fisher Scientific (3 mentions) Hcclm3, by Thermo Fisher Scientific (3 mentions) Smmc 7721, by Thermo Fisher Scientific (3 mentions) Hek293t, by National Collection of Authenticated Cell Cultures (3 mentions) Streptomycin, by Thermo Fisher Scientific (3 mentions) Penicillin, by Thermo Fisher Scientific (3 mentions)

56 protocols using plc prf 5

1

Hepatocyte and Hepatocellular Carcinoma Cell Culture

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Human normal hepatocyte cell line (LO2) and HCC cell lines (HepG2, Hep3B, HLF and PLC/PRF/5) which were purchased from National Collection of Authenticated Cell Cultures (https://www.cellbank.org.cn) were maintained in the DMEM medium (Gibco, Wuhan, China) with 10% heat-inactivated fetal bovine serum (FBS, GibcoBRL) and antibiotics of 1% streptomycin and penicillin at 37° C in an atmosphere of 5% CO2.
Wang Z., Embaye K.S., Yang Q., Qin L., Zhang C., Liu L., Zhan X., Zhang F., Wang X, & Qin S. (2021). A Novel Metabolism-Related Signature as a Candidate Prognostic Biomarker for Hepatocellular Carcinoma. Journal of Hepatocellular Carcinoma, 8, 119-132.
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2

HCC Cell Culture Protocols

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The HCC cell lines, HepG2 and PLC/PRF/5, were provided by the National Collection of Authenticated Cell Cultures (Shanghai, China). All cells were grown in DMEM containing 10% of fetal bovine serum (FBS) at 37°C in a 5% CO2 atmosphere.
Zhang Y., Qin S., Chao J., Luo Y., Sun Y, & Duan J. (2022). The In-Vitro Antitumor Effects of AST-3424 Monotherapy and Combination Therapy With Oxaliplatin or 5-Fluorouracil in Primary Liver Cancer. Frontiers in Oncology, 12, 885139.
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3

Liver Cancer Tissue and Cell Line Samples

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Tumor or normal liver tissues, and peripheral blood samples were obtained from the patients who underwent liver resections for HCC, or benign liver lesions such as hemangioma and focal nodule hyperplasia (FNH) in our institute with the informed consent of the patients. Tissue samples were preserved by frozen or fixed in 10% formalin. These were approved by the Research Ethics Committee of Huashan Hospital, Fudan University (KY2023-594, Shanghai, China).
5–8 weeks male C57BL/6 or nude mice were purchased from Gempharmatech Co., Ltd (Nanjing, China. https://www.gempharmatech.com/) and kept in a SPF (specific pathogen free) room at 25 °C, 50–60% moisture. Human HCC cell line HepG2, PLC/PRF/5(refer to PLC below), Huh7 and murine HCC cell line Hepa1-6 were obtained from National Collection of Authenticated Cell Cultures (Shanghai, China. https://www.cellbank.org.cn/). All human and mouse liver cancer cells were cultured in DMEM with 10% FBS, glutamine and 1% penicillin–streptomycin (Gibco) at 37 °C and 5% CO2. Public data resources included transcriptional data of TCGA-LIHC and mass spectrometry proteomics data of CHCC-HBV (Chinese HCC patients with HBV infection), which were downloaded from GDC Data Portal (https://portal.gdc.cancer.gov/) or from additional table S1 from ref [16 (link)].
Shen X.T., Xie S.Z., Zheng X., Zou T.T., Hu B.Y., Xu J., Liu L., Xu Y.F., Wang X.F., Wang H., Wang S., Zhu L., Yu K.K., Zhu W.W., Lu L., Zhang J.B., Chen J.H., Dong Q.Z., Yang L.Y, & Qin L.X. (2024). Cirrhotic-extracellular matrix attenuates aPD-1 treatment response by initiating immunosuppressive neutrophil extracellular traps formation in hepatocellular carcinoma. Experimental Hematology & Oncology, 13, 20.
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4

Cell Culture of PLC/PRF/5 and SMMC-7721

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PLC/PRF/5 and SMMC-7721 cells were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in high-glucose Dulbecco's modified Eagle's medium (Hyclone; GE Healthcare, Chicago, IL, USA) added with 10% fetal bovine serum (FBS; Hyclone; GE Healthcare) and 1% penicillin/streptomycin at 37°C containing 5% CO2. Cells were passaged when they reached 80% confluency and used after the third passage.
Wang H., Zhang C., Chi H, & Meng Z. (2018). Synergistic anticancer effects of bufalin and sorafenib by regulating apoptosis associated proteins. Molecular Medicine Reports, 17(6), 8101-8110.
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5

HCC Cell Lines Cultivation and Treatment

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Human HCC cell lines PLC/PRF/5 and Huh7 were purchased from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. HCC-LM3 cells were taken from our laboratory stocks. All the HCC cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Corning, Inc., Corning, NY, USA) with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and were maintained in an incubator with 5% CO2 at 37 °C. Sorafenib and Bafilomycin A1 were obtained from Selleck Chemicals (Houston, TX, USA). Primary antibodies used in this study were listed below: MTX1 (15529-1-AP, Proteintech, Wuhan, China), CISD1 (16006-1-AP, Proteintech, Wuhan, China), Beclin1 (11306-1-AP, Proteintech, Wuhan, China), LC3 (14600-1-AP, Proteintech, Wuhan, China), GAPDH (60004-1-Ig, Proteintech, Wuhan, China), β-Actin (sc-8432, Santa Cruz Biotechnology, USA) and FLAG (abs830005, Absin, China).
Li L., Yu S., Hu Q., Hai Y, & Li Y. (2021). Genome-scale CRISPRa screening identifies MTX1 as a contributor for sorafenib resistance in hepatocellular carcinoma by augmenting autophagy. International Journal of Biological Sciences, 17(12), 3133-3144.
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6

Culturing Human Liver Cell Lines

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The normal human liver cell line LO2, and HCC cell lines HepG2, Huh7, Hep3B, PLC/PRF/5, MHCC-97H, HCCLM3, SMMC-7721 were purchased from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. LO2, Huh7, MHCC-97H, HCCLM3 were cultured in DMEM (Invitrogen), supplemented with 1% antibiotics (GIBCO) and 10% fetal bovine serum (GEMINI). The HepG2, Hep3B, PLC/PRF/5 cell lines were cultured in MEM (Invitrogen) supplemented with 10% FBS and 1% antibiotics. The SMMC-7721 was cultured in RPMI1640 (Invitrogen) with 1% Penicillin-Streptomycin and 10% FBS. All cells were cultured in 5% CO2 humid atmosphere at 37 °C.
Yan X., Zhu Z., Xu S., Yang L.N., Liao X.H., Zheng M., Yang D., Wang J., Chen D., Wang L., Liu X., Liu J., Chen R.H., Zhen Zhou X., Ping Lu K, & Liu H. (2017). MicroRNA-140-5p inhibits hepatocellular carcinoma by directly targeting the unique isomerase Pin1 to block multiple cancer-driving pathways. Scientific Reports, 7, 45915.
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7

Cell Culture Protocols for Hepatocyte and HCC Lines

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Human hepatocyte L02 cell line and HCC cell lines including HepG2, Huh-7 and PLC/PRF/5 were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). L02, HepG2 Cells were cultured in RPMI-1640 medium and Huh-7, PLC/PRF/5 cells were cultured in high glucose DMEM medium supplemented with 10% foetal bovine serum (FBS) and 1% penicillin-streptomycin (Biological Industries, Kibbutz Beit Haemek, Israel) in a 5% CO2, humidified atmosphere at 37°C.
Geng N., Jin Y., Li Y., Zhu S, & Bai H. (2020). AKR1B10 Inhibitor Epalrestat Facilitates Sorafenib-Induced Apoptosis and Autophagy Via Targeting the mTOR Pathway in Hepatocellular Carcinoma. International Journal of Medical Sciences, 17(9), 1246-1256.
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8

Culturing Human Hepatocyte Cell Lines

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Human HCC cell lines Huh7, PLC/PRF/5, HL7702 and non-transformed human hepatocyte-derived cell line HHL-5 were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Vero cells were maintained in the Department of Microbiology, Harbin Medical University, Harbin, China. Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal calf serum (FCS) (Biological Industries, Israel) and 1% penicillin and streptomycin at 37° C with 5% CO2.
Wu X., Yang Y., Huang Y., Chen Y., Wang T., Wu S., Tong L., Wang Y., Lin L., Hao M., Zhong Z.H., Zhang F, & Zhao W. (2018). RNA-binding protein AUF1 suppresses miR-122 biogenesis by down-regulating Dicer1 in hepatocellular carcinoma. Oncotarget, 9(19), 14815-14827.
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9

Cell Culture Protocols for Hepatocellular Carcinoma

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Hep G2, Huh‐7, SMMC‐7721 and PLC/PRF/5 cells were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). These cells were cultured in Dulbecco's modified Eagle's medium (Hep G2, Huh‐7 and PLC/PRF/5) or RPMI 1640 medium (SMMC‐7721) supplemented with 10% foetal bovine serum (HyClone) and 100 U/mL penicillin and streptomycin in a humidified incubator with 5% CO2 and 95% air.
Bai T., Lei P., Zhou H., Liang R., Zhu R., Wang W., Zhou L, & Sun Y. (2019). Sigma‐1 receptor protects against ferroptosis in hepatocellular carcinoma cells. Journal of Cellular and Molecular Medicine, 23(11), 7349-7359.
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10

Cell Culture Conditions for PLC/PRF/5 and Huh-7

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PLC/PRF/5 and Huh-7 cells were purchased from the Type Culture Collection of the Chinese Academy of Sciences. Huh-7 cultured in RPMI-1640 (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS. PLC/PRF/5 cells cultured in minimum essential medium (MEM; Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS. All cells were maintained in a humidified atmosphere maintained at 37°C with 5% CO2.
Ma Y., Ma M., Ma L., Zhang F., Liu Y, & Ma X. (2019). Downregulation of miR-552 in hepatocellular carcinoma inhibits cell migration and invasion, and promotes cell apoptosis via RUNX3. Experimental and Therapeutic Medicine, 18(5), 3829-3836.
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