Mm 1s cells

Five human multiple myeloma cell lines, Delta47, U266, KMS-5, RPMI8226, and MM.1 S cells as well as three human T-cell lymphoma cell lines, Karpas 299, H9, and HUT102 cells were supplied by American Type Culture Collection (Manassas, VA) and maintained in RPMI 1640 (Gibco BRL, Tokyo, Japan) with 10% heat-inactivated fetal bovine serum (Sigma, St. Louis, MO).

PBMCs and BMMCs were obtained from the UAMS Myeloma Center Tissue Biorepository and Procurement Core. Human MM1.S cells and mouse RAW264.7 cells were purchased from the American Type Culture Collection (ATCC). Human OPM2 and H929 cells were provided by Siegfried Janz (Medical College of Wisconsin, Milwaukee, Wisconsin, USA). MM1.S, OPM2, and H929 cell lines were cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific) containing 10% fetal calf serum (FCS) (Gibco, Thermo Fisher Scientific), 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco, Thermo Fisher Scientific). The RAW264.7 cell line was cultured in DMEM (Gibco, Thermo Fisher Scientific) containing 10% fetal calf serum (FCS) (Gibco, Thermo Fisher Scientific), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Thermo Fisher Scientific).

Multiple myeloma MM.1S cells were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA) and cryopreserved in a liquid nitrogen tank in the laboratory. According to the culture method recommended by ATCC, the MM.1S cells were cultured in RPMI-1640 medium containing 10% FBS at 37 °C with 5% CO₂. When MM.1S cells were growing well and in logarithmic growth phase, they were used for subsequent experiments.