Cecropin a

Manufactured by Bachem

Sourced in Japan, Switzerland

Cecropin A is a laboratory-produced peptide. It is a naturally occurring antimicrobial peptide isolated from the Cecropia moth. Cecropin A demonstrates broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria.

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Lab products found in correlation

N decane, by Fujifilm (1 mentions) Potassium chloride kcl, by Nacalai Tesque (1 mentions) 3 morpholinopropane 1 sulfonic acid mops, by Nacalai Tesque (1 mentions) Ll 37, by AnaSpec (1 mentions) Milli q system, by Merck Group (1 mentions) 1 2 dioleoyl sn glycero 3 phosphoethanolamine dope, by Avanti Polar Lipids (1 mentions) 1 2 dioleoyl sn glycero 3 phospho 1 rac glycerol dopg, by Avanti Polar Lipids (1 mentions) Polymyxin b, by Merck Group (1 mentions) Etest strips, by bioMérieux (1 mentions) Polymyxin b, by bioMérieux (1 mentions)

2 protocols using cecropin a

Optimizing Lipid Membrane Composition for Antimicrobial Peptide Characterization

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In this study, we used the following reagents: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE; Avanti Polar Lipids,
AL, USA); 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG; Avanti Polar Lipids); n-decane (Wako Pure Chemical Industries, Ltd., Osaka, Japan); 3-morpholinopropane-1-sulfonic
acid (MOPS, Nacalai Tesque, Kyoto, Japan); potassium chloride (KCl;
Nacalai Tesque); clavanin A (GenScript); cecropin A (BACHEM); pardaxin
(abcam); magainin 1 (AnaSpec Inc); SL-37 (Cosmo Bio Co., Ltd.); and
LL-37 (AnaSpec Inc.). DOPE and DOPG were melted in n-decane at a concentration of 10 mg/mL, and we obtained a lipid mixture
of DOPE/DOPG (3:1 mol/mol). The composition of the measurement buffer
was regulated so that it became 200 mM KCl, 10 mM MOPS, and pH 7.0
in the Milli-Q system (Millipore, Billerica, MA, USA). The clavanin
A, cecropin A, pardaxin, magainin 1, SL-37, and LL-37 powders were
dissolved in the measurement buffer and preserved at 4 °C.

Saigo N., Izumi K, & Kawano R. (2019). Electrophysiological Analysis of Antimicrobial Peptides in Diverse Species. ACS Omega, 4(8), 13124-13130.

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Determination of Minimum Inhibitory Concentrations

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MIC determination in liquid medium was done by performing microdilution assays. Briefly, selected bacterial strains were grown overnight at 25°C and 180 rpm in LB and washed once in saline solution.
The bacteria were inoculated into 96-well microtiter plates at a starting OD600 of 0.01 in a total volume of 200 μl LB supplemented with different concentrations of polymyxin B (Sigma, St. Louis, Missouri, USA) and in 150 μl LB supplemented with different concentrations of cecropin A and cecropin B (Bachem AG, Bubendorf, Switzerland). The MIC for a given strain was defined as the concentration at which no visible growth was observable after 24 h of incubation at 25°C and 300 rpm. The assay was carried out at least twice per strain at independent time points. MIC determination for polymyxin B on solid medium was performed using Etest strips (bioMérieux SA, Marcy l'Etoile, France) as described previously (Cullen et al., 2015) , with nutrient agar (NA) plates and incubation at 25°C, and interpreted according to manufacturer's instructions. The assay was performed twice per strain.

Kupferschmied P., Chai T., Flury P., Blom J., Smits T.H., Maurhofer M, & Keel C. (2016). Specific surface glycan decorations enable antimicrobial peptide resistance in plant-beneficial pseudomonads with insect-pathogenic properties. Environmental microbiology, 18(11).

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