Antibodies used in Western blotting and immunofluorescence were as follows: mouse monoclonal antibody 8D5 against β-DG (Novocastra); mouse monoclonal antibody IIH6 against α-DG (Millipore); affinity-purified goat polyclonal antibody against the α-DG core protein (AP-074G-C) [23] (link); mouse monoclonal antibody NCL-Hamlet against dysferlin (Novocastra); rat monoclonal antibody against mouse F4/80 (BioLegend); rabbit polyclonal antibody against collagen I (AbD serotec); rabbit polyclonal antibody against albumin (DAKO); mouse monoclonal antibody against caveolin-3 (BD Transduction Laboratories); rabbit polyclonal antibody against caveolin-3 (Abcam); and rabbit polyclonal antibody against Trim72 (MG53) (Abcam).
Rat monoclonal antibody against mouse f4 80
Manufactured by BioLegend
The Rat monoclonal antibody against mouse F4/80 is a laboratory tool used for the detection and analysis of the F4/80 antigen, which is expressed on the surface of mouse macrophages. This antibody can be utilized in various immunological techniques, such as flow cytometry and immunohistochemistry, to identify and characterize macrophage populations.
Automatically generated - may contain errors
2 protocols using rat monoclonal antibody against mouse f4 80
1
Antibody Validation for Western Blotting and Immunofluorescence
2
Antibody Characterization for Dystroglycan Research
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Antibodies for western blotting and immunofluorescence were as follows: mouse monoclonal antibody 8D5 against β-DG (Novocastra); mouse monoclonal antibody IIH6 against α-DG (Millipore); rat monoclonal antibody against mouse F4/80 (BioLegend); rabbit polyclonal antibody against collagen I (AbD Serotec); mouse monoclonal antibody against FLAG tag (Sigma); and rabbit polyclonal antibody against c-Myc tag (Santa Cruz). Rabbit polyclonal antibody against LARGE was raised using recombinant human LARGE protein expressed in E. coli. Antisera were purified by Melon gel IgG purification kit (Pierce). Rat monoclonal antibody against the α-DG core protein (3D7) was generated from a α-DG-Fc fusion protein52 (link).
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