Cxcl12α

Manufactured by Thermo Fisher Scientific

CXCL12α is a recombinant protein produced by Thermo Fisher Scientific. It is a member of the CXC chemokine family and functions as a chemoattractant for various cell types.

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Lab products found in correlation

4 protocols using cxcl12α

Procurement and Preparation of Growth Factors

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All GFs and chemokines were purchased in their mature forms, highly pure (>95% pure), carrier-free, and lyophilized^{35 (link)}. VEGF-A121, VEGF-A165, PlGF-1, PlGF-2, PDGF-AA, PDGF-BB, PDGF-CC, PDGF-DD, FGF-1, FGF-2, FGF-6, FGF-7, FGF-9, FGF-10, FGF-18, BMP-2, BMP-3, BMP-4, BMP-7, β-NGF, NT-3, BDNF, IGF-1, IGF-2, HB-EGF, CXCL-11, and CXCL-12α were purchased from PeproTech. CXCL-12γ was purchased from R&D systems. Except for PDGF-DD and BMP-7, which were produced in eukaryotic cells, all GFs were produced in Escherichia coli and thus were not glycosylated. All GFs were reconstituted and stored according to the provider’s instructions to regain full activity and prevent loss of protein.

Ishihara J., Ishihara A., Fukunaga K., Sasaki K., White M.J., Briquez P.S, & Hubbell J.A. (2018). Laminin heparin-binding peptides bind to several growth factors and enhance diabetic wound healing. Nature Communications, 9, 2163.

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Rescue Protocol for Intestinal Ischemia-Reperfusion

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In rescue experiments, mice were treated with 5 μg RSPO3 (R&D systems #4120RS025CF) in 100 μl PBS (Kannan et al, 2013 (link)), or CXCL12α (PeproTech #250‐20A) in PBS at a dose of 50 μg/kg of body weight (BW; Garnica et al, 2005 (link)) by retro‐orbital injection 30 min before intestinal ischemia. Distal jejunum was harvested after I/R at 18.5 h (WB) or 24 h (H&E, IHC) for further analysis.

Tan C., Norden P.R., Yu W., Liu T., Ujiie N., Lee S.K., Yan X., Dyakiv Y., Aoto K., Ortega S., De Plaen I.G., Sampath V, & Kume T. (2023). Endothelial FOXC1 and FOXC2 promote intestinal regeneration after ischemia–reperfusion injury. EMBO Reports, 24(7), e56030.

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CXCL12α Loading and Release from MSCM-NF

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CXCL12α (100, 200, and 300 ng) (PeproTech) in PBS was mixed with LNP-Cas9/MSCM-NF (50 μg) and incubated for 1 hour at 37°C. The amount of unbound CXCL12α was quantified by enzyme-linked immunosorbent assay (ELISA) (RayBiotech) to evaluate CXCL12α loading. CXCL12α-loaded MSCM-NF (50 μg) was incubated in 500 μl of RPMI with 10% FBS at 37°C. The supernatant (100 μl) was collected and supplemented with fresh RPMI medium (100 μl) at each time point. The amount of CXCL12α released was quantified by ELISA. For other in vitro and in vivo studies, LNP-Cas9 RNP was first loaded onto MSCM-NF and then CXCL12α was incorporated.

Ho T.C., Kim H.S., Chen Y., Li Y., LaMere M.W., Chen C., Wang H., Gong J., Palumbo C.D., Ashton J.M., Kim H., Xu Q., Becker M.W, & Leong K.W. (2021). Scaffold-mediated CRISPR-Cas9 delivery system for acute myeloid leukemia therapy. Science Advances, 7(21), eabg3217.

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Chemokine Protocols for SPR and Chemotaxis

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Recombinant human chemokines used in SPR experiments (CXCL1, CXCL2, CXCL3, CXCL4, CXCL5, CXCL6, CXCL7, CXCL8, CXCL9, CXCL10, CXCL11, CXCL12-α, CXCL12-β, CXCL13, CXCL14, CXCL16, CCL1, CCL2, CCL3, CCL4, CCL5, CCL7, CCL8, CCL11, CCL14 (66 aa), CCL14 (72 aa), CCL15, CCL16, CCL17, CCL18, CCL19, CCL20, CCL21, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CX3CL1 and XCL1), or in chemotaxis experiments (CXCL12-α, CCL2, CCL3, CCL5 and CCL7) were from Peprotech and were reconstituted in 1x PBS with 0.1% BSA as a carrier protein.

González-Motos V., Jürgens C., Ritter B., Kropp K.A., Durán V., Larsen O., Binz A., Ouwendijk W.J., Lenac Rovis T., Jonjic S., Verjans G.M., Sodeik B., Krey T., Bauerfeind R., Schulz T.F., Kaufer B.B., Kalinke U., Proudfoot A.E., Rosenkilde M.M, & Viejo-Borbolla A. (2017). Varicella zoster virus glycoprotein C increases chemokine-mediated leukocyte migration. PLoS Pathogens, 13(5), e1006346.

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