Transpack packaging extract
The Transpack Packaging Extract is a laboratory equipment product from Agilent Technologies. It is designed for the extraction and purification of analytes from a variety of sample matrices. The core function of this product is to facilitate the sample preparation process.
Lab products found in correlation
14 protocols using transpack packaging extract
Evaluating Perillaldehyde-Induced Mutagenesis
Efficient Lambda Phage DNA Packaging
Cisplatin-induced Mutation Frequency Assay
Extraction and Phage Recovery from Lung DNA
Isolation of 6-TG-Resistant Mutants
Genomic DNA Extraction and Phage Packaging
In Vitro Mutagenicity Assay with GPT
Quantifying gpt Mutation Frequency
the small intestine mucosa by means of a RecoverEase DNA Isolation Kit (Agilent
Technologies, Santa Clara, CA, USA), and lambda EG10 phages were recovered with Transpack
Packaging Extract (Agilent Technologies). Escherichia coli YG6020 were
infected with the recovered phages, plated on M9 salt plates containing chloramphenicol
(Cm) and 6-thioguanine (6-TG), and then incubated for 72–90 h at 37°C. This incubation
enabled selection of colonies harboring a plasmid carrying both the gene for
chloramphenicol acetyltransferase and a mutated gpt gene.
gpt-Mutant frequency was calculated by dividing the number of mutated
colonies growing on agar plates containing Cm and 6-TG by the number of colonies growing
on agar plates containing Cm alone. The mutants exhibiting the 6-TG-resistant phenotype
were cultured overnight at 37°C in Luria–Bertani broth containing 25 µg/mL Cm, harvested
by centrifugation (7000 rpm, 10 min), and then stored at −80°C. A 739-bp DNA fragment
containing gpt was amplified by means of the polymerase chain reaction
and sequenced as described previously12 (link),13 (link)).
Identification of gpt Mutations in Rescued Phages
Identifying Mutations in gpt Gene
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