His k63

Manufactured by Thermo Fisher Scientific

His-K63 is a laboratory equipment product designed for the purification and detection of proteins containing the K63-linked polyubiquitin chains. It is used in various research applications involving the study of protein ubiquitination and cellular signaling pathways.

Automatically generated - may contain errors

Lab products found in correlation

Profection, by Promega (2 mentions) Viafect, by Promega (2 mentions) Flag keap1, by Addgene (2 mentions) Ha k48, by Addgene (2 mentions) Ha k63, by Addgene (2 mentions) Gfp polyq74, by Addgene (2 mentions) Gfp p62, by Addgene (2 mentions) Gfp nrf2, by Addgene (2 mentions) Gfp keap1, by Addgene (2 mentions) Myc nrf2, by Addgene (2 mentions) Flag nrf2, by Addgene (2 mentions) Flag ulk1, by Addgene (2 mentions) Effectene, by Qiagen (2 mentions)

2 protocols using his k63

Molecular Mechanisms of NRF2 Regulation

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GFP‐polyQ74 (#40262), GFP‐p62 (#38277), GFP‐NRF2 (#21549), GFP‐KEAP1 (#28025), Flag‐KEAP1 (#28023), Myc‐NRF2 (#21555), Flag‐NRF2 (#36971), HA‐K48 (#17605), HA‐K63 (#17606), Flag‐ULK1 (#27636) were procured from Addgene. GFP‐TRIM16 and Flag‐TRIM16 were cloned as described previously (Chauhan et al, 2016). Myc‐TRIM16, Myc‐TRIM16 deletion constructs, and His‐K63‐UB were generated using Gateway cloning strategy as per standard protocol (Invitrogen).
The siRNA for NRF2 and p62 were purchased from Sigma, and TRIM16, Ubb, Ube2n siRNA were from Dharmacon. For overexpression experiments, HEK293T cells were transfected using calcium phosphate method as per the manufacturer's instructions (Profection, Promega). Other cells are transfected using Effectene (Qiagen) or Viafect (Promega) or Interference (Polyplus) as per the manufacturer's instruction.

Jena K.K., Kolapalli S.P., Mehto S., Nath P., Das B., Sahoo P.K., Ahad A., Syed G.H., Raghav S.K., Senapati S., Chauhan S, & Chauhan S. (2018). TRIM16 controls assembly and degradation of protein aggregates by modulating the p62‐NRF2 axis and autophagy. The EMBO Journal, 37(18), e98358.

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Characterization of NRF2-KEAP1 Pathway

Cited 1 time

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GFP-polyQ74 (#40262), GFP-p62 (#38277), GFP-NRF2 (#21549), GFP-KEAP1 (#28025), Flag-KEAP1 (#28023), Myc-NRF2 (#21555), Flag-NRF2 (#36971), HA-K48 (#17605), HA-K63 (#17606), Flag-ULK1 (#27636) were procured from Addgene. GFP-TRIM16 and Flag-TRIM16 were cloned as described previously (Chauhan et al, 2016 (link)). Myc-TRIM16, Myc-TRIM16 deletion constructs, and His-K63-UB were generated using Gateway cloning strategy as per standard protocol (Invitrogen).
The siRNA for NRF2 and p62 were purchased from Sigma, and TRIM16, Ubb, Ube2n siRNA were from Dharmacon. For overexpression experiments, HEK293T cells were transfected using calcium phosphate method as per the manufacturer’s instructions (Profection, Promega). Other cells are transfected using Effectene (Qiagen) or Viafect (Promega) or Interference (Polyplus) as per the manufacturer’s instruction.

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