Tenascin

Manufactured by Thermo Fisher Scientific

Sourced in Australia, United States

Tenascin is a large extracellular matrix glycoprotein that plays a role in cell adhesion and migration. It is involved in the regulation of various cellular processes, including embryonic development, tissue repair, and tumor progression. Tenascin functions as a modulator of cell-matrix interactions and can influence cell behavior through its interaction with cell surface receptors.

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Lab products found in correlation

Fibronectin, by Thermo Fisher Scientific (2 mentions) Fibronectin, by Merck Group (1 mentions) Tenascin, by Merck Group (1 mentions) Collagen type 1, by Merck Group (1 mentions) Collagen type 1, by Thermo Fisher Scientific (1 mentions) Vitronectin, by Thermo Fisher Scientific (1 mentions) Laminin, by Thermo Fisher Scientific (1 mentions) Laminin 5, by Merck Group (1 mentions) Collagen 4, by RayBiotech (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) β actin antibody, by Merck Group (1 mentions) β catenin, by BD (1 mentions) E cadherin, by BD (1 mentions) Gm130, by BD (1 mentions)

3 protocols using tenascin

ECM Protein Deposition Quantification

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Fibronectin, type I collagen, tenascin and perlecan deposition into the ECM was measured by ECM ELISA using optimised monoclonal mouse-anti human perlecan (2 μg/ml) (ThermoFisher Scientific), type I collagen (2 μg/ml)(Sigma-Aldrich), Fibronectin (0.5 μg/ml) (Merck, Bayswater, Australia) and tenascin (0.5 μg/ml) (Sigma-Aldrich) antibodies as previously described by Kuo et al. (2011) [34 (link)].

Rutting S., Papanicolaou M., Xenaki D., Wood L.G., Mullin A.M., Hansbro P.M, & Oliver B.G. (2018). Dietary ω-6 polyunsaturated fatty acid arachidonic acid increases inflammation, but inhibits ECM protein expression in COPD. Respiratory Research, 19, 211.

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Mesenchymal Stem Cell Characterization

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The bone-marrow derived human mesenchymal stem cells (hMSCs), its culture media, fetal bovine serum (FBS), L-Alanyl-L-Glutamine, Insulin-like Growth Factor-1, basic Fibroblast Growth Factor, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide were obtained from American Type Culture Collection (ATCC, VA, USA). Acridine orange and propidium iodide working solution, ECM cell adhesion array kit (colorimetric; ECM540) for collagen-I, II, and IV, fibronectin, laminin, tenascin, and vitronectin were obtained from ThermoFisher Scientific (MA, USA). All other chemicals stated were either purchase from Sigma (MO, USA) or Thermo Fisher Scientific (MA, USA).

Kumar S., Stokes JA I.I.I., Dean D., Rogers C., Nyairo E., Thomas V, & Mishra M.K. (2017). Biphasic organo-bioceramic fibrous composite as a biomimetic extracellular matrix for bone tissue regeneration. Frontiers in bioscience (Elite edition), 9, 192-203.

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Antibody Sources for Cell Biology

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Here are the sources of our antibodies: E-cadherin, β-catenin, and GM130 (BD Biosciences, San Jose, CA); cleaved caspase-3 (Cell Signaling Technology, Beverly, MA); tenascin (Thermo Fisher Scientific, Pittsburgh, PA), laminin V (Millipore, Billerica, MA); collagen IV (Raybiotech, Norcross, GA); and β-actin antibody (Sigma-Aldrich, St. Louis, MO).

Choi P.W., Yang J., Ng S.K., Feltmate C., Muto M.G., Hasselblatt K., Lafferty-Whyte K., JeBailey L., MacConaill L., Welch W.R., Fong W.P., Berkowitz R.S, & Ng S.W. (2015). Loss of E-cadherin disrupts ovarian epithelial inclusion cyst formation and collective cell movement in ovarian cancer cells. Oncotarget, 7(4), 4110-4121.

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